Hostname: page-component-586b7cd67f-t7fkt Total loading time: 0 Render date: 2024-11-24T17:48:20.917Z Has data issue: false hasContentIssue false
  • English
  • Français

Water availability and metabolomic profiles of Epicoccum nigrum and Sarophorum palmicola grown in solid substrate fermentation systems

Published online by Cambridge University Press:  18 March 2005

DAVID ALDRED ,
JULIA PENN  and
NARESH MAGAN
DAVID ALDRED
Affiliation:
Applied Mycology Group, Institute of BioScience and Technology, Cranfield University, Silsoe, Bedford MK45 4DT, [email protected]
JULIA PENN
Affiliation:
Cubist Pharmaceuticals (UK) Ltd., 545 Ipswich Road, Slough, Berkshire, SL1 4EQ, UK.
NARESH MAGAN
Affiliation:
Applied Mycology Group, Institute of BioScience and Technology, Cranfield University, Silsoe, Bedford MK45 4DT, [email protected]
Get access

Abstract

There has been interest in using environmental screening procedures for enhancing the metabolomic production profiles by fungi which are of pharmaceutical interest. This study examined two ecologically distinct species, Epicoccum nigrum and Sarophorum palmicola, in solid substrate fermentation systems over a range of water availability conditions (water activity, aW). Total secondary metabolite profiles were obtained by HPLC + diode array detection after 14 days incubation on cereal-based substrates in relation to four aW treatments. Temporal studies (24 d, E. nigrum and 18 d, S. palmicola) showed that metabolite production profiles varied markedly between the two fungi. For E. nigrum, metabolite production generally increased with reduction in aW, and was optimal in the range 0.99 - 0.97. In contrast to this, for S. palmicola, metabolite production was restricted to the highest aW level used, 0.998, and declined to zero at 0.99 aW. Statistical analysis revealed that time, aW and their interactions were significant in all cases (p<0.001). The potential for using ecophysiological stresses for enhancing natural product discovery is discussed.

Keywords

Metabolomic profileswater availabilitysolid substrate fermentation
Type
Original Article
Information
Mycologist , Volume 19 , Issue 1 , February 2005 , pp. 18 - 23
Copyright
© 2005 Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)