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VNTR polymorphism in the ITS1 region (rDNA) in isolates of a Nodulisporium species

Published online by Cambridge University Press:  18 December 2002

Gonzalo PLATAS ,
Jon D. POLISHOOK  and
Fernando PELÁEZ
Gonzalo PLATAS
Affiliation:
Centro de Investigación Básica, Merck Sharp & Dohme de España, S. A. Josefa Valcarcel 38, 28027 Madrid, Spain. E-mail: [email protected]
Jon D. POLISHOOK
Affiliation:
Microbiology, Merck Research Laboratories, P.O. Box 2000, Rahway, NJ 07065, USA.
Fernando PELÁEZ
Affiliation:
Centro de Investigación Básica, Merck Sharp & Dohme de España, S. A. Josefa Valcarcel 38, 28027 Madrid, Spain. E-mail: [email protected]
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Abstract

A Variable Number of Tandem Repeats (VNTR) polymorphism has been identified in the ITS1 region of the rDNA in 13 strains belonging to a species of Nodulisporium producing the novel indole diterpene nodulisporic acid. The number of tandem repeats found in these isolates varies from 2, 4 or 5 repetitions, and produces three length morphs in the ITS1 rDNA, spanning a length difference of 217, 308 and 352 nucleotides. Repeated units are rather variable, and they encompass three tandem repeated motifs of 11 nucleotides. In all the individuals, the variants are positioned in the same physical order, following the distribution of the most parsimonious tree. This suggests that this sequence have been enlarged by slipped-strand mispairing occurrences.

Type
Research Article
Information
Mycological Research , Volume 106 , Issue 11 , November 2002 , pp. 1293 - 1298
Copyright
© The British Mycological Society 2002

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