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PCR as a tool for the early detection and diagnosis of common bunt in wheat, caused by Tilletia tritici

Published online by Cambridge University Press:  18 December 2002

Lone JOSEFSEN  and
Solveig K. CHRISTIANSEN
Lone JOSEFSEN
Affiliation:
Plant Research Department, Risø National Laboratory, Post Office Box 49, DK-4000 Roskilde, Denmark. E-mail: [email protected]
Solveig K. CHRISTIANSEN
Affiliation:
Plant Research Department, Risø National Laboratory, Post Office Box 49, DK-4000 Roskilde, Denmark. E-mail: [email protected]
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Abstract

A method for the early detection and diagnosis of common bunt, caused by Tilletia tritici, has been developed. The technique is based on a nested PCR amplification of DNA from T. tritici in infected plant inflorescence tissue at the stage of internode elongation. Primers amplifying a 212 bp fragment in T. tritici were designed on the basis of sequences in the ITS2 region in the ribosomal RNA genes. Disease diagnosis obtained with nested PCR amplification correlated well with the conventional disease diagnosis, which involves manual observation of disease symptoms at maturity. With the present method, wheat varieties can be screened for resistance towards common bunt at the first node stage. There was no variation between the ITS2 region from T. tritici, T. laevis, T. controversa, and T. bromi and the primers are therefore not species specific. The PCR diagnosis method has only been validated for common bunt caused by T. tritici, but it is likely also to be applicable to T. laevis and T. controversa.

Type
Research Article
Information
Mycological Research , Volume 106 , Issue 11 , November 2002 , pp. 1287 - 1292
Copyright
© The British Mycological Society 2002

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