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Morphological and molecular characterisation of Geosmithia putterillii, G. pallida comb. nov. and G. flava sp. nov., associated with subcorticolous insects

Published online by Cambridge University Press:  21 September 2004

Miroslav KOLAŘÍK ,
Alena KUBÁTOVÁ ,
Sylvie PAŽOUTOVÁ  and
Petr šRŮTKA
Miroslav KOLAŘÍK
Affiliation:
Department of Botany, Faculty of Science, Charles University, Benátská 2, 128 0, Praha 2, Czech Republic. E-mail: [email protected] Institute of Microbiology CAS, 142 20 Praha 4, Czech Republic.
Alena KUBÁTOVÁ
Affiliation:
Department of Botany, Faculty of Science, Charles University, Benátská 2, 128 0, Praha 2, Czech Republic. E-mail: [email protected]
Sylvie PAŽOUTOVÁ
Affiliation:
Institute of Microbiology CAS, 142 20 Praha 4, Czech Republic.
Petr šRŮTKA
Affiliation:
Faculty of Forestry, Czech University of Agriculture, Kamýcká 129, 165 21 Praha 6, Czech Republic.
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Abstract

Geosmithia putterillii is an anamorphic fungus with connections to bark beetles. Genetic variability of 89 isolates traditionally grouped in G. putterillii and G. lavendula isolated from different geographical regions from subcorticolous insects and from other unspecific substrata was assessed using RAPD, sequencing of the ITS region (ITS1-5.8SrDNA-ITS2) and morphological characters. RAPD analysis revealed eight distinct groups. One group was represented by G. lavendula type strain and showed no relations to any other isolate. Five RAPD-types with similar ITS sequences and phenotype were related to the ex-type strain of Penicillium pallidum (generally given as a synonym of G. putterillii). Because of unique phylogenetic position and a phenotype markedly different from G. putterillii, the new combination G. pallida is made here. For another group of isolates formerly identified as G. putterillii the new species G. flava is described based on a characteristic RAPD-type, a unique ITS sequences and a different phenotype. These newly recognized species are stable in culture and with worldwide distribution.

Type
Research Article
Information
Mycological Research , Volume 108 , Issue 9 , September 2004 , pp. 1053 - 1069
Copyright
© The British Mycological Society 2004

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