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Grouping of lignin degrading corticioid fungi based on RFLP analysis of 18S rDNA and ITS regions

Published online by Cambridge University Press:  01 August 1999

ANETA DRESLER-NURMI ,
SEPPO KAIJALAINEN ,
KRISTINA LINDSTRÖM  and
ANNELE HATAKKA
ANETA DRESLER-NURMI
Affiliation:
Department of Applied Chemistry and Microbiology, P.O. Box 56, Biocenter 1, FIN-00014 University of Helsinki, Finland
SEPPO KAIJALAINEN
Affiliation:
Department of Applied Chemistry and Microbiology, P.O. Box 56, Biocenter 1, FIN-00014 University of Helsinki, Finland
KRISTINA LINDSTRÖM
Affiliation:
Department of Applied Chemistry and Microbiology, P.O. Box 56, Biocenter 1, FIN-00014 University of Helsinki, Finland
ANNELE HATAKKA
Affiliation:
Department of Applied Chemistry and Microbiology, P.O. Box 56, Biocenter 1, FIN-00014 University of Helsinki, Finland
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Abstract

Twelve wood decaying species of Phlebia and Phanerochaete were analysed by RFLP (restriction fragment length polymorphism) analysis of an 18S rRNA gene fragment and an ITS region. The data obtained by different restriction endonucleases were used to construct phenograms based on the UPGMA algorithm. PCR-RFLP within the 18S rRNA gene was sufficient to distinguish between Phlebia species but did not show variation among Phanerochaete spp. ITS region amplified from Phlebia spp. varied in length from 570–745 bp. The smallest ITS fragment was amplified from P. subcretacea and the longest from P. hydnoides. The size of ITS fragments amplified from Phanerochaete spp. was uniform (635 bp), except for the ITS from two P. sanguinea strains (690 bp). RFLP analysis within ITS amplified from Phanerochaete spp. distinguished between them. Results from PCR-RFLP of 18S rRNA and ITS strongly suggest that Phiebia gigantea is closely related to Phanerochaete. Morphological characteristics (lack of clamp connections in hymenium, well developed subiculum) further support this hypothesis.

Type
Research Article
Information
Mycological Research , Volume 103 , Issue 8 , August 1999 , pp. 990 - 996
Copyright
The British Mycological Society 1999

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