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Genetic variability of Beauveria bassiana associated with the Coffee Berry Borer Hypothenemus hampei and other insects

Published online by Cambridge University Press:  18 December 2002

Alvaro GAITAN ,
Ana M. VALDERRAMA ,
Gabriel SALDARRIAGA ,
Patricia VELEZ  and
Alex BUSTILLO
Alvaro GAITAN
Affiliation:
National Coffee Research Center, CENICAFE, Plan Alto, Chinchiná, Caldas, Colombia. E-mail: [email protected]
Ana M. VALDERRAMA
Affiliation:
National Coffee Research Center, CENICAFE, Plan Alto, Chinchiná, Caldas, Colombia. E-mail: [email protected]
Gabriel SALDARRIAGA
Affiliation:
National Coffee Research Center, CENICAFE, Plan Alto, Chinchiná, Caldas, Colombia. E-mail: [email protected]
Patricia VELEZ
Affiliation:
National Coffee Research Center, CENICAFE, Plan Alto, Chinchiná, Caldas, Colombia. E-mail: [email protected]
Alex BUSTILLO
Affiliation:
National Coffee Research Center, CENICAFE, Plan Alto, Chinchiná, Caldas, Colombia. E-mail: [email protected]
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Abstract

Genetic variability of 95 isolates of Beauveria bassiana from different geographical regions and hosts was assessed using RAPDs and ITS-RFLP molecular markers in order to characterize its genetic variability. Clusterings were obtained using UPGMA and Principal Coordinates Analysis. Four groups were identified with RAPDs. A 930 bp fragment was amplified with primers ITS1 and PN16, and polymorphisms were observed with AluI and MspI, generating two main clusters. Results indicate low genetic variability present in the Colombian B. bassiana population, no association to host type or geographic locality, and a population structure that is not clonal. This characterization sets criteria for determination of bio-insecticide potentiality, introduction of foreign strains and improvement of entomopathogens within an IPM program.

Type
Research Article
Information
Mycological Research , Volume 106 , Issue 11 , November 2002 , pp. 1307 - 1314
Copyright
© The British Mycological Society 2002

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