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Genetic transformation of ectomycorrhizal fungi mediated by Agrobacterium tumefaciens

Published online by Cambridge University Press:  09 May 2002

Alejandro G. PARDO ,
Mubashir HANIF ,
Marjatta RAUDASKOSKI  and
Markus GORFER
Alejandro G. PARDO
Affiliation:
Department of Biosciences, Division of Plant Physiology, P.O.B. 56, University of Helsinki, Helsinki F-00014, Finland. Programa de Investigación en Interacciones Biológicas, Centro de Estudios e Investigaciones y Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Roque Sáenz Peña 180, (B1876BXD) Bernal, Provincia de Buenos Aires, Argentina. Present address: INRA, Centre de Nancy, UMR IaM, 5480 Champenoux, France.
Mubashir HANIF
Affiliation:
Department of Biosciences, Division of Plant Physiology, P.O.B. 56, University of Helsinki, Helsinki F-00014, Finland.
Marjatta RAUDASKOSKI
Affiliation:
Department of Biosciences, Division of Plant Physiology, P.O.B. 56, University of Helsinki, Helsinki F-00014, Finland.
Markus GORFER
Affiliation:
Department of Biosciences, Division of Plant Physiology, P.O.B. 56, University of Helsinki, Helsinki F-00014, Finland. Present address: Ecowork Lab coratoria, Längenfeldgasse 27, 1120 Vienna, Austria.
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Abstract

A technique was developed for transforming the ectomycorrhiza-forming basidiomycetes Suillus bovinus, Hebeloma cylindrosporum, and Paxillus involutus based on Agrobacterium tumefaciens-mediated T-DNA transfer. The selection marker employed was the Shble gene conferring resistance to phleomycin under control of the Schizophyllum commune GPD promoter and terminator. Transformants from all three investigated species were shown by PCR to contain the GPDScP-Shble-GPDScT construct, although the fate of the foreign DNA (integrated vs episomal, single-copy vs multi copy) could not be determined. The mycorrhiza formed between S. bovinus Bler transformants and Pinus sylvestris did not reveal any differences from those formed with untransformed Suillus bovinus.

Type
Research Article
Information
Mycological Research , Volume 106 , Issue 2 , February 2002 , pp. 132 - 137
Copyright
© The British Mycological Society 2002

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