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Cryopreservation of the ectomycorrhizal mushroom Cantharellus cibarius

Published online by Cambridge University Press:  18 December 2002

Eric DANELL  and
Gunnar FLYGH
Eric DANELL
Affiliation:
Department of Forest Mycology and Pathology, Swedish University of Agricultural Sciences, Box 7026, SE-750 07 Uppsala, Sweden Museum of Evolution, Botany section, Uppsala University, Norbyv.16, SE-752 36 Uppsala, Sweden
Gunnar FLYGH
Affiliation:
Department of Forest Mycology and Pathology, Swedish University of Agricultural Sciences, Box 7026, SE-750 07 Uppsala, Sweden Department of Veterinary Medical Chemistry, Swedish University of Agricultural Sciences, Box 575, SE-751 23 Uppsala, Sweden
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Abstract

Cryopreservation of ectomycorrhizal fungi is often difficult, but needed for patent deposits and for enabling experiments on model strains with constant characteristics. In this study we report a protocol for successful cryopreservation of the ectomycorrhizal mushroom Cantharellus cibarius, together with a brief summary of some unsuccessful attempts. A slow freezing rate (0.3°C min−1) of agar cubes carrying the mycelium, and a gentle addition of the cryoprotectants sorbitol and DMSO were important features. This protocol was successfully repeated by ATCC who used it to deposit a patented strain of C. cibarius, and therefore the protocol should also be tested for other ectomycorrhizal basidiomycetes.

Type
Research Article
Information
Mycological Research , Volume 106 , Issue 11 , November 2002 , pp. 1340 - 1342
Copyright
© The British Mycological Society 2002

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