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Phylogenetic analysis of the bluestain fungus Ophiostoma minus based on partial ITS rDNA and β-tubulin gene sequences

Published online by Cambridge University Press:  05 August 2004

Caroline GORTON ,
Seong Hwan KIM ,
Béatrice HENRICOT ,
Joan WEBBER  and
Colette BREUIL
Caroline GORTON
Affiliation:
Plant Pathology Department, The Royal Horticultural Society, Wisley, Woking, Surrey GU23 6QB, UK.
Seong Hwan KIM
Affiliation:
Department of Microbiology, Dankook University, Cheonan, Chungnam, 330-714 Korea. E-mail: [email protected]
Béatrice HENRICOT
Affiliation:
Plant Pathology Department, The Royal Horticultural Society, Wisley, Woking, Surrey GU23 6QB, UK.
Joan WEBBER
Affiliation:
Forestry Commission Research Agency, Alice Holt Lodge, Farnham, Surrey GU10 4LH, UK.
Colette BREUIL
Affiliation:
Department of Wood Science, University of British Columbia, 4041-2424 Main Mall, Vancouver, BC, V6T1Z4, Canada.
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Abstract

In an attempt to clarify the relationship between fungi classified as Ophiostoma minus, but of different geographic origins and mating systems, sequencing of the 5.8S and ITS 2 rDNA, and β-tubulin gene was carried out. The β-tubulin gene was highly informative, supporting the sub-division of O. minus into two groups based on geographic origin. Furthermore, isolates previously classified as O. pseudotsugae were confirmed as being clearly distinct from O. minus. However, sequencing did not reveal any polymorphisms between isolates with homothallic as compared to heterothallic mating systems. This was supported by crosses using methylbenzamidazole-2-yl carbamate nuclear markers which showed that hybridisation between isolates of different mating systems was possible. However, we propose that different mating systems may still signal a divergence of isolates of O. minus.

Type
Research Article
Information
Mycological Research , Volume 108 , Issue 7 , July 2004 , pp. 759 - 765
Copyright
© The British Mycological Society 2004

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