Hostname: page-component-78c5997874-94fs2 Total loading time: 0 Render date: 2024-11-02T18:51:36.241Z Has data issue: false hasContentIssue false
  • English
  • Français

Microinjected antisense Inf24 oligonucleotides inhibit appressorium development in Uromyces

Published online by Cambridge University Press:  01 December 1998

FRANÇOIS BARJA ,
ARY CORRÊA JR ,
R. C. STAPLES  and
H. C. HOCH
FRANÇOIS BARJA
Affiliation:
Laboratoire de Microbiologie générale, Sciences III, 30 quai E-Ansemet, 1211 Genève 4, Switzerland
ARY CORRÊA JR
Affiliation:
Departamento de Microbiologia, UFMG, Av. Antônio Carlos 6627, Belo Horizonte-M.G., C.P. 486, CEP-31270-901, Brazil
R. C. STAPLES
Affiliation:
Department of Plant Pathology, Cornell University, New York State Agricultural Experiment Station, Geneva, New York 14456, U.S.A.
H. C. HOCH
Affiliation:
Department of Plant Pathology, Cornell University, New York State Agricultural Experiment Station, Geneva, New York 14456, U.S.A.
Get access

Abstract

Germlings of Uromyces appendiculatus respond to topographical stimuli and develop appressoria. During this period several genes are expressed, one of which is Inf24. Germlings were microinjected with a sense and antisense fragment of the ORF of Inf24. Sense (Inf24-S), 5′-ATG AGT GCT TCA TCG CAG CAG CAG-3′ fragment did not affect germling response to the topographical stimuli and the developed appressoria. Microinjection of antisense (Inf24-RC), 5′-CTG CTG CTG CGA TGA AGC ACT CAT-3′ blocked gene transcription and appressoria were rarely formed. Injection of Inf24-RC into already formed appressoria did not inhibit continued development of subsequent infection structures, e.g. penetration peg, sub-stomatal vesicle.

Type
Research Article
Information
Mycological Research , Volume 102 , Issue 12 , December 1998 , pp. 1513 - 1518
Copyright
© The British Mycological Society 1998

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)