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Use of Sputter-Deposited 316L Stainless Steel Ultrathin Films for Microbial Influenced Corrosion Studies

Published online by Cambridge University Press:  01 January 1992

P.A. Suci ,
A.J. Pedraza ,
M.J. Godbole  and
G.G. Geesey
P.A. Suci
Affiliation:
Montana State University, Department of Microbiology, Bozeman, MT
A.J. Pedraza
Affiliation:
University of Tennessee, Department of Materials Science, Knoxville, TN
M.J. Godbole
Affiliation:
University of Tennessee, Department of Materials Science, Knoxville, TN
G.G. Geesey
Affiliation:
Montana State University, Department of Microbiology, Bozeman, MT
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Abstract

Ultra thin films (12nm) were sputter deposited onto cylindrical germanium internal reflection elements pre-coated with a thin (2nm) layer of Cr2O3. Two crystals were inserted into Circle cell flow-through chambers and mounted on the optical bench of an Fourier Transform Infrared (FT-IR) spectrometer. One chamber was maintained as a sterile control while the other was sequentially inoculated with four bacterial species: Pseudomonas aeruginosa, Bacillus subtillis, Hafnia alvei, and Desulfovibrio gigas, in that order. The water absorption band (1640cm−1) was monitored and used to follow the deterioration of the ultra thin films. In this respect, the sterile control and inoculated films exhibited only slight differences during the 1000h course of the experiment. Assay of the visible biofilm that had accumulated on the surface of the inoculated crystal after 1000h revealed that the film incorporated viable cells from all four strains.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 294: Symposium V – Scientific Basis for Nuclear Waste Management XVI , 1992 , 381
Copyright
Copyright © Materials Research Society 1993

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References

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