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Published online by Cambridge University Press: 31 January 2011
Anodic aluminum oxide (AAO) template was prepared by using anodizing voltage step-decreasing method after two-step oxide method. Based on AAO template, Fe nanowires arrays were electrochemically deposited. Fe nanowires were coated by chitosan. Fe nanowires/chitosan was synthesized by glutaraldehyde as cross-linking reagent. By crosslinking α-human chorionic gonadotropin (α-HCG), biological probes with Fe nanowires/chitosan/antibody were prepared. An easy operating, easy taking and rapid reacting magnetic detecting system was developed after optimizing the geometry parameters of detect coil. Different concentration samples with 1, 2 and 5 g(Fe)/L were detected. The results show that the sensitivity of system is 0.2 g(Fe)/L and can be improve better.