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Stimulating living cells with air DBD plasma

Published online by Cambridge University Press:  16 May 2012

P. Favia ,
D. Pignatelli ,
G. Dilecce ,
B. R. Pistillo ,
M. Nardulli  and
R. Gristina
P. Favia
Affiliation:
Department of Chemistry, University of Bari “A. Moro”, Italy CNR-Institute of Inorganic Methodologies and Plasmas, IMIP-CNR, Bari, Italy Plasma Solution Srl, Spin Off dell’Università di Bari “A. Moro”, Italy
D. Pignatelli
Affiliation:
Department of Chemistry, University of Bari “A. Moro”, Italy
G. Dilecce
Affiliation:
CNR-Institute of Inorganic Methodologies and Plasmas, IMIP-CNR, Bari, Italy
B. R. Pistillo
Affiliation:
Department of Chemistry, University of Bari “A. Moro”, Italy
M. Nardulli
Affiliation:
Department of Chemistry, University of Bari “A. Moro”, Italy
R. Gristina
Affiliation:
CNR-Institute of Inorganic Methodologies and Plasmas, IMIP-CNR, Bari, Italy
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Abstract

Cells positioned at the bottom of a Petri dish were exposed, in a home-made plasma source, to pulsed Dielectric Barrier Discharges operated in air in order to investigate the effect of the plasma species on their viability and growth. Processes with different number of pulse, respectively 1,3,9 and 27 pulse, were performed to study the influence on viability and cell growth of two different cell lines, Saos 2 and NHDF. Atmospheric air discharges applied on the two selected cell lines have shown an effect strongly dependent on cell type. At certain doses we have measured increased activity of the NHDF fibroblasts cell line. On the other side, an inhibition of cell adhesion and growth on the Saos 2 osteoblastoma cell line, directly dependent on the plasma doses, was clear. This study shows that by properly tuning the dose of exposure of cells to air plasma it is possible to induce both positive and negative effects on cell growth, that would be useful in several branches of Medicine.

Keywords

biomedicaldielectricbiological
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1469: Symposium WW – Plasma Processing and Diagnostics for Life Sciences , 2012 , mrss12-1469-ww04-03
Copyright
Copyright © Materials Research Society 2012

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References

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