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Site-selective immobilization of streptavidin on enzymatically biotinylated bacterial magnetic particles

Published online by Cambridge University Press:  01 February 2011

Yoshiaki Maeda ,
Tomoko Yoshino ,
Haruko Takeyama ,
Masaaki Takahashi ,
Harumi Ginya ,
Junko Asahina ,
Hideji Tajima  and
Tadashi Matsunaga
Yoshiaki Maeda
Affiliation:
[email protected], Tokyo University of Agriculture and Technology, Department of Biotechnology, 2-24-16, Naka-cho, Koganei, 184-8588, Japan
Tomoko Yoshino
Affiliation:
[email protected], Tokyo University of Agriculture and Technology, Department of Biotechnology, 2-24-16, Naka-cho, Koganei, 184-8588, Japan
Haruko Takeyama
Affiliation:
[email protected], Tokyo University of Agriculture and Technology, Department of Biotechnology, 2-24-16, Naka-cho, Koganei, 184-8588, Japan
Masaaki Takahashi
Affiliation:
[email protected], Precision System Science Co., Ltd., Japan, 88 Kamihongo, Matsudo, Chiba, 271-0064, Japan
Harumi Ginya
Affiliation:
[email protected], Precision System Science Co., Ltd., Japan, 88 Kamihongo, Matsudo, Chiba, 271-0064, Japan
Junko Asahina
Affiliation:
[email protected], Precision System Science Co., Ltd., Japan, 88 Kamihongo, Matsudo, Chiba, 271-0064, Japan
Hideji Tajima
Affiliation:
[email protected], Precision System Science Co., Ltd., Japan, 88 Kamihongo, Matsudo, Chiba, 271-0064, Japan
Tadashi Matsunaga
Affiliation:
[email protected], Tokyo University of Agriculture and Technology, Department of Biotechnology, 2-24-16, Naka-cho, Koganei, 184-8588, Japan
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Abstract

Biotinylated magnetic nanoparticles were constructed by displaying biotin acceptor peptide (BAP) on the surfaces of bacterial magnetic particles (BacMPs) synthesized by Magnetospirillum magneticum AMB-1. Both BAP and green fluorescent protein (GFP) were fused to Mms13 that was isolated from BacMP membranes. The localization of the fusion protein, BAP-Mms13-GFP, was confirmed by fluorescence analysis. BacMPs that expressed BAP-Mms13-GFP (BAP/GFP-BacMPs) were extracted from bacterial cells and incubated with biotin and Escherichia coli biotin ligase. The in vitro biotinylation of BAP/GFP-BacMPs was confirmed using alkaline phosphatase (ALP)-streptavidin detection. The conjugation system developed in this study provides a method for producing biotin- or streptavidin-labeled magnetic nanoparticles without the use of a crosslinker reagent. Various functional materials can be immobilized site-selectively onto these uniquely designed BacMPs. By combining this site-selective biotinylation technology and protein display methodology, increasingly innovative and attractive magnetic nano-materials can be constructed.

Keywords

biomaterialmagneticparticulate
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1094: Symposium DD – From Biological Materials to Biomimetic Material Synthesis , 2008 , 1094-DD07-19
Copyright
Copyright © Materials Research Society 2008

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References

1. Yoshino, T. and Matsunaga, T., Appl. Environ. Microbiol. 72, 465 (2006).Google Scholar
2. Maruyama, K., Takeyama, H., Nemoto, E., Tanaka, T., Yoda, K. and Matsunaga, T., Biotechnol. Bioeng. 87, 687 (2004).Google Scholar
3. Nakagawa, T., Hashimoto, R., Maruyama, K., Tanaka, T., Takeyama, H. and Matsunaga, T., Biotechnol. Bioeng. 94, 862 (2006).Google Scholar
4. Nakagawa, T., Maruyama, K., Takeyama, H. and Matsunaga, T., Biosens. Bioelectron. 22, 2276 (2007).Google Scholar
5. Matsunaga, T., Maeda, Y., Yoshino, T., Takeyama, H., Takahashi, M., Ginya, H., Aasahina, J. and Tajima, H., Anal. Chim. Acta. 597, 331 (2007).Google Scholar
6. Schatz, P. J., Nat. Biotechnol. 11, 1138 (1993).Google Scholar
7. Matsunaga, T., Sakaguchi, T. and Tadokoro, F., Appl. Microbiol. Biotechnol. 35, 651 (1991).Google Scholar
8. Boer, E. d., Rodriguez, P., Bonte, E., Krijgsveld, J., Katsantoni, E., Heck, A. and Grosveld, F., Strouboulis, J., Proc. Natl. Acad. Sci. U.S.A. 100, 7480 (2003).Google Scholar
9. Howarth, M., Takao, K., Hayashi, Y. and Ting, A. Y., Proc. Natl. Acad. Sci. U.S.A. 102, 7583 (2005).Google Scholar
10. Nakamura, M., Mie, M., Funabashi, H., Yamamoto, K., Ando, J. and Kobatake, E., Anal. Biochem. 352, 61 (2006).Google Scholar