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Reptation of Microtubules in F-Actin Networks : Effects of Filament Stiffness and Network Topology on Reptation Dynamics

Published online by Cambridge University Press:  15 February 2011

Jagesh V. Shah ,
Lisa A. Flanagan ,
David Bahk  and
Paul A. Janmey
Jagesh V. Shah
Affiliation:
Harvard-MIT Division of Health Sciences and Technology, Cambridge MA 02138, [email protected]
Lisa A. Flanagan
Affiliation:
Div. of Experimental Medicine, Brigham and Women's Hospital, Harvard Medical School, 221 Longwood Ave., LMRC 301, Boston, MA 02115
David Bahk
Affiliation:
Div. of Experimental Medicine, Brigham and Women's Hospital, Harvard Medical School, 221 Longwood Ave., LMRC 301, Boston, MA 02115
Paul A. Janmey
Affiliation:
Div. of Experimental Medicine, Brigham and Women's Hospital, Harvard Medical School, 221 Longwood Ave., LMRC 301, Boston, MA 02115
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Abstract

The thermally driven motions of fluorescently labeled microtubules embedded in a network of filamentous actin polymers are analysed as the diffusion of a rod-like polymer within a virtual tube formed by the surrounding semiflexible actin filaments. The apparent diffusion constant parallel to the tube scales with the inverse of the microtubule length and the magnitude is consistant with diffusion through a medium with a viscosity of approximately 10 centipoise. Introduction of crosslinks between the actin filaments does not alter the diffusion of the microtubules in the actin network.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 489: Symposium K – Materials Science of the Cell , 1997 , 27
Copyright
Copyright © Materials Research Society 1998

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