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Rapid and Sensitive Detection of Nano-fluidically Trapped Protein Biomarkers

Published online by Cambridge University Press:  21 November 2014

Nandhinee Radha Shanmugam ,
Anjan Panneer Selvam ,
Thomas W. Barrett ,
Steve Kazmierczak  and
Shalini Prasad
Nandhinee Radha Shanmugam
Affiliation:
Department of Bioengineering, University of Texas at Dallas, Richardson, TX 75080, U.S.A.
Anjan Panneer Selvam
Affiliation:
Department of Bioengineering, University of Texas at Dallas, Richardson, TX 75080, U.S.A.
Thomas W. Barrett
Affiliation:
Department of Veterans Affairs, Oregon Health & Science University, Portland, OR, U.S.A.
Steve Kazmierczak
Affiliation:
Department of Pathology, Oregon Health & Science University, Portland, OR, U.S.A.
Shalini Prasad
Affiliation:
Department of Bioengineering, University of Texas at Dallas, Richardson, TX 75080, U.S.A.
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Abstract

In this work, we demonstrate the label-free and ultrasensitive detection of troponin-T, cardiac biomarker using nanoporous membrane integrated on a microelectrode sensor platform. The nanoporous membrane allows for spatial confinement of the protein molecules. Antigen interaction with thiol immobilized antibody perturbs the electrical double layer. Charge perturbations are recorded as impedance change at low frequency using the principle of electrochemical impedance spectroscopy (EIS). The measured impedance change is used to quantitatively determine the concentration of troponin-T in tested sample. We have shown that sensitivity of sensor for troponin-T to be 1pg/mL. The accuracy and reliability of this sensor was tested by comparing the experimental troponin-T concentration values with a commercially available electrochemiluminescence assay measured with Roche Elecsys analyzer. Using this technique we were successful in detecting protein biomarkers in whole blood, human serum, and ionic buffers. This technology provides a robust analytical platform for rapid and sensitive detection of protein biomarkers, thus establishing this technology as an ideal candidate for biomarker screening in clinical settings.

Keywords

nanoscaleporositysensor
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1686: Symposium V – Micro- and Nanofluidic Systems for Materials Synthesis, Device Assembly and Bioanalysis , 2014 , mrss14-1686-v09-13
Copyright
Copyright © Materials Research Society 2014 

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References

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