Hostname: page-component-586b7cd67f-dsjbd Total loading time: 0 Render date: 2024-11-28T10:40:14.348Z Has data issue: false hasContentIssue false
  • English
  • Français

Preparation and Electrochemical Characterization of DNA-modified Nanocrystalline Diamond Films

Published online by Cambridge University Press:  11 February 2011

Wensha Yang ,
Orlando Auciello ,
James E. Butler ,
Wei Cai ,
John A. Carlisle ,
Jennifer E. Gerbi ,
Dieter M. Gruen ,
Tanya Knickerbocker ,
Tami L. Lasseter  and
John N. Russell Jr
...Show all authors
Wensha Yang
Affiliation:
Department of Chemistry, University of Wisconsin-Madison, 1101 University Avenue Madison, WI 53706
Orlando Auciello
Affiliation:
Materials Science Division Argonne National Laboratory 9700 S. Cass Ave. Argonne, Illinois 60439
James E. Butler
Affiliation:
Naval Research Laboratory 4555 Overlook Ave. S.W. Washington, DC 20375
Wei Cai
Affiliation:
Department of Chemistry, University of Wisconsin-Madison, 1101 University Avenue Madison, WI 53706
John A. Carlisle
Affiliation:
Materials Science Division Argonne National Laboratory 9700 S. Cass Ave. Argonne, Illinois 60439
Jennifer E. Gerbi
Affiliation:
Materials Science Division Argonne National Laboratory 9700 S. Cass Ave. Argonne, Illinois 60439
Dieter M. Gruen
Affiliation:
Materials Science Division Argonne National Laboratory 9700 S. Cass Ave. Argonne, Illinois 60439
Tanya Knickerbocker
Affiliation:
Department of Chemistry, University of Wisconsin-Madison, 1101 University Avenue Madison, WI 53706
Tami L. Lasseter
Affiliation:
Department of Chemistry, University of Wisconsin-Madison, 1101 University Avenue Madison, WI 53706
John N. Russell Jr
Affiliation:
Naval Research Laboratory 4555 Overlook Ave. S.W. Washington, DC 20375
Lloyd M. Smith
Affiliation:
Department of Chemistry, University of Wisconsin-Madison, 1101 University Avenue Madison, WI 53706
Robert J. Hamers
Affiliation:
Department of Chemistry, University of Wisconsin-Madison, 1101 University Avenue Madison, WI 53706
Get access

Abstract

Nanocrystalline diamond thin films of sub-micron thickness have been covalently modified with DNA oligonucleotides. Quantitative studies of hybridization of surface-bound oligonucleotides with fluorescently tagged complementary and non-complementary oligonucleotides were performed. The results show no detectable nonspecific adsorption, with extremely good selectivity between matched and mismatched sequences. Impedance spectroscopy measurements were made of DNA-modified boron-doped nanocrystalline diamond films. The results show that exposure to non-complementary sequences induce only small changes in impedance, while complementary DNA sequences produce a pronounced decrease in impedance. The combination of high stability, selectivity, and the ability to directly detect DNA hybridization via electrical means suggest that diamond may be an ideal substrate for continuously-monitoring biological sensors.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 737 , 2002 , F4.4
Copyright
Copyright © Materials Research Society 2003

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Buriak, J. M. Chemical Reviews, 102, 1271–1308.(2002)Google Scholar
Strother, T., Hamers, R. J., Smith, L. M. Nucleic Acids Research, 28, 3535–3541.(2000)Google Scholar
Strother, T., Cai, W., Zhao, X., Hamers, R. J., Smith, L. M. J. Am Chem. Soc., 122, 1205–1209.(2000)Google Scholar
4. Strother, T., Knickerbocker, T., Russell, J. N. Jr, Butler, J. E., Smith, L. M., Hamers, R. J. Langmuir, 18, 968–971.(2002)Google Scholar
5. Brockman, J. M., Frutos, A. G., Corn, R. M. J. Am. Chem. Soc., 121, 8044–8051.(1999)Google Scholar
6. Souteyrand, E., Cloarec, J. P., Martin, J. R., Wilson, C., Lawrence, I., Mikkelsen, S., Lawrence, M. F. Journal of Physical Chemistry B, 101, 2980–2985.(1997)Google Scholar
7. Chrisey, L. A., Lee, G. U., O'Ferrall, E. O. Nucleic Acids Res., 24, 3031–3039.(1996)Google Scholar
8. Knickerbocker, T., Strother, T., Schwartz, M. P., Smith, L. M., Hamers, R. J. Langmuir, submitted for publication. (2002)Google Scholar
9. Swain, G. M., Ramesham, M. Analytical Chemistry, 65, 345–351.(1993)Google Scholar
10. Wei, J., Yates, J. T. Jr, Critical Reviews in Surface Chemistry, 5, 1–71.(1995)Google Scholar
11. Rotter, S. Israel Journal of Chemistry, 38, 135–140.(1998)Google Scholar
12. May, P. W. Phil. Trans. Royal Soc. London A, 358, 473–495.(2000)Google Scholar
13. Corrigan, T.D., Krauss, A.R., Gruen, D.M., Auciello, O., and Chang, R.P.H., Mater. Res. Soc. Symp. Proc., 593, 233–236 (2000).Google Scholar
14. Butler, J. E., Windischmann, H. MRS Bulletin, 23, 22–27.(1998)Google Scholar
15. Gruen, D. M. Annual Reviews of Materials Science, 29, 211–259.(1999)Google Scholar
16. Bhattacharyya, S., Auciello, O., Birrell, J., Carlisle, J. A., Curtiss, L. A., Goyette, A. N., Gruen, D. M., Krauss, A. R., Schlueter, J., Sumant, A., and Zapol, P., Appl. Phys. Lett. 79, 1441 (2001).Google Scholar
17. Thoms, B. D., Owens, M. S., Butler, J. E., Spiro, C. Applied Physics Letters., 65, 2957. (1994)Google Scholar
18. Yang, W., Butler, J. E., Cai, W., Carlisle, J., Gruen, D., Knickerbocker, T., Russell, J. N. Jr, Smith, L. M., Hamers, R. J. Nature-Materials, in press. (December 2002)Google Scholar
19. Cai, W., Peck, J. R., Yang, W., Van der Weide, D., Hamers, R. J. in preparation. (2002)Google Scholar
20. Kelley, S. O., Barton, J. K. Science, 283, 375–381.(1999)Google Scholar
21. Treadway, C. R., Hill, M. G., Barton, J. K. Chemical Physics, 281, 409–428.(2002)Google Scholar
22. Lee, T.-Y., Shim, Y.-B. Analytical Chemistry, 73, 5629–5632. (2001)Google Scholar