Hostname: page-component-745bb68f8f-b95js Total loading time: 0 Render date: 2025-01-27T15:39:22.275Z Has data issue: false hasContentIssue false
  • English
  • Français

Potentiometric Detection of DNA Molecules Hybridization Using Gene Field Effect Transistor and Intercalator

Published online by Cambridge University Press:  01 February 2011

Toshiya Sakata ,
Hidenori Otsuka  and
Yuji Miyahara
Toshiya Sakata
Affiliation:
Biomaterials Center, National Institute for Materials Science, 1–1 Namiki, Tsukuba, Ibaraki 305–0044, Japan
Hidenori Otsuka
Affiliation:
Biomaterials Center, National Institute for Materials Science, 1–1 Namiki, Tsukuba, Ibaraki 305–0044, Japan
Yuji Miyahara
Affiliation:
Biomaterials Center, National Institute for Materials Science, 1–1 Namiki, Tsukuba, Ibaraki 305–0044, Japan
Get access

Abstract

We propose a new concept of a gene field effect transistor (FET) for detection of allele specific oligonucleotide hybridization, which is in principle based on charge density change at the gate insulator. The electrical characteristics of the gene FET were found to shift after specific binding of biomolecules at the surface of the gate insulator. Allele specific oligonucleotide hybridization and reaction between double-stranded DNA and intercalator were successfully detected with gene FETs because they have intrinsic charges in an aqueous solution. Ability to discriminate single nucleotide polymorphism (SNP) was also examined using the gene FET. Our results show that control of hybridization temperature and utilization of intercalator lead to more precise SNP analysis using the gene FET.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 782: Symposium A – Micro- and Nanosystems , 2003 , A7.6
Copyright
Copyright © Materials Research Society 2004

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

1. Zarrinkar, P.P. et al. Arrays of arrays for high-throughput gene expression profiling. Genome Res. 11, 1256–1261 (2001).Google Scholar
2. Carter, T.A. et al. Chipping away at complex behavior: transcriptome/phenotype correlations in the mouse brain. Physiol. Behav. 73, 849–857 (2001).Google Scholar
3. Lindblad-Toh, K. et al. Loss-of-heterozygosity analysis of small-cell lung carcinomas using single-nucleotide polymorphism arrays. Nat. Biotechnol. 18, 1001–1005 (2000).Google Scholar
4. Stephen, , Fodor, P. A. et al. Multiplexed biochemical assays with biological chips. Nature 364, 555–556 (1993).Google Scholar
5. Xiang, C.C. et al. Amine-modified random primers to label probes for DNA microarrays. Nat. Biotechnol. 20, 738 - 742 (2002).Google Scholar
6. Zhao, X. et al. Ultrasensitive DNA detection using highly fluorescent bioconjugated nanoparticles. J. Am. Chem. Soc. 125, 11474–11475 (2003).Google Scholar
7. Cutler, D.J. et al. High-throughput variation detection and genotyping using microarrays. Genome Res. 11, 1913–1925 (2001).Google Scholar
8. David, J. L. et al. Expression monitoring by hybridization to high-density oligonucleotide arrays. Nat. Biotechnol. 14, 1645–1680 (1996).Google Scholar
9. Takenaka, S. et al. DNA sensing on a DNA probe-modified electrode using ferrocenylnapthalenediimide as the electrochemically active ligand. Anal. Chem. 72, 1334–1341 (2000).Google Scholar
10. Souteyrand, E. et al. Direct detection of the hybridization of synthetic homo-oligomer DNA sequences by field effect. J. Phys. Chem. B 101, 2980–2985 (1997).Google Scholar
11. Otto, F. & Tsou, K.G. A comparative study of DAPI, DIPI, and Hoechst 33258 and 33342 as chromosomal DNA stains. Stain Technology 60, 7 (1985).Google Scholar
12. Boger, D. L. et al. A simple, high-resolution method for establishing DNA binding affinity and sequence selectivity. J. Am. Chem. Soc. 123, 5878–5891 (2001).Google Scholar
13. Kajiyama, T. et al. Genotyping on a thermal gradient DNA chip. Genome Res. 13, 467–475 (2003).Google Scholar