Hostname: page-component-586b7cd67f-g8jcs Total loading time: 0 Render date: 2024-11-28T05:13:08.285Z Has data issue: false hasContentIssue false
  • English
  • Français

Porous Scaffolds Consisting of Collagen, Chondroitin Sulfate, and Hydroxyapatite with Enhanced Biodegradable Resistance for Cartilage Regeneration

Published online by Cambridge University Press:  14 March 2011

H. Kaneda ,
T. Ikoma ,
T. Yoshioka ,
M. Nishi ,
R. Matsumoto ,
T. Uemura ,
J. S. Cross  and
J. Tanaka
H. Kaneda
Affiliation:
Department of Metallurgy and Ceramics Science, Tokyo Institute of Technology, Tokyo, Japan
T. Ikoma
Affiliation:
Department of Metallurgy and Ceramics Science, Tokyo Institute of Technology, Tokyo, Japan
T. Yoshioka
Affiliation:
Department of Metallurgy and Ceramics Science, Tokyo Institute of Technology, Tokyo, Japan
M. Nishi
Affiliation:
Nanosystem Research Institute (NRI), National Institute of Advanced Industrial Science and Technology (AIST), Ibaraki, Japan.
R. Matsumoto
Affiliation:
Nanosystem Research Institute (NRI), National Institute of Advanced Industrial Science and Technology (AIST), Ibaraki, Japan.
T. Uemura
Affiliation:
Nanosystem Research Institute (NRI), National Institute of Advanced Industrial Science and Technology (AIST), Ibaraki, Japan.
J. S. Cross
Affiliation:
Department of Metallurgy and Ceramics Science, Tokyo Institute of Technology, Tokyo, Japan
J. Tanaka
Affiliation:
Department of Metallurgy and Ceramics Science, Tokyo Institute of Technology, Tokyo, Japan
Get access

Abstract

Porous scaffolds of alkaline-soluble collagen including nanocomposite particles of chondroitin sulfate and low crystalline hydroxyapatite for cartilage regeneration were fabricated by freeze-drying and thermal dehydration treatments; porous collagen scaffolds were also synthesized as a reference. The scaffolds were cross-linked using glutaraldehyde (GA) vapor treatment in order to enhance biodegradable resistance. Microstructural observation with scanning electron microscope indicated that the scaffolds with and without GA cross-linkage had open pores between 130 to 200 μm in diameter and well-interconnected pores of 10 to 30 μm even after cross-linkage. In vitro biodegradable resistance to collagenase was significantly enhanced by GA cross-linking of the scaffolds. All these results suggest that the GA cross-linked scaffolds consisting of collagen, chondroitin sulfate, and low crystalline hydroxyapatite have suitable microporous structures and long-term biochemical stability for cartilage tissue engineering.

Keywords

biomaterialcompositetissue
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1301: Symposium V/NN/OO/PP – Soft Matter, Biological Materials and Biomedical Materials—Synthesis, Characterization and Applications , 2011 , mrsf10-1301-oo07-06
Copyright
Copyright © Materials Research Society 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

[1] Chu, C. R., Coutts, R. D., Yoshioka, M., Harwood, F. L., Monosov, A. Z. and Amiel, D., J Biomed Mater Res 29 (1995), pp. 1147–1154.Google Scholar
[2] Grande, D. A., Halberstadt, C., Naughton, G., Schwartz, R. and Ryhana, M., J Biomed Mater Res 34 (1997), pp. 211–220.Google Scholar
[3] Ohyabu, Y., Adegawa, T., Yoshioka, T., Ikoma, T., Shinozaki, K., Uemura, T., Tanaka, J., Journal of Biomaterials Science, 20, 1861–1874, (2009).Google Scholar
[4] Ma, Lie, Gao, Changyo, Mao, Zhengwei, Zhou, Jie, Shen, Jiacong, Hu, Xueqing, Han, Chunmao, Biomaterials, 24, 4833–4841, (2003).Google Scholar
[5] Jorge-Herrero, E, Fernandez, P, Turnay, J, Olmo, N., Calero, P., Garcia, R., Freile, I., Castillo-Olivares, J. L., 20, 539–45, (1999).Google Scholar
[6] Ikoma, T., Muneta, T., Tanaka, J., Key Eng. Mater., Vol. 192-195, 487–490, (2001).Google Scholar
[7] Adegawa, T., Ohyabu, Y., Uemura, T., Yoshioka, T, Shinozaki, K., Tanaka, J., Key Eng. Mater., Vol. 396-398, pp707–710 (2009).Google Scholar
[8] Choi, Y. S., Hong, S. R., Lee, Y. M., Song, K. W., Park, M. H., Nam, Y. S., Biomaterials, 20, 409–417, (1999).Google Scholar
[9] Olde Damink, LHH, Dijkstra, PJ, Van Luyu, MJA, Van Wachem, PB, Nieuwenhuis, P, Feijen, J, J Mater Sci Mater Med, 6, 460–472, (1995).Google Scholar
[10] Park, Si-Nae, Park, Jong-Chul, Kim, Hea Ok, Song, Min Jung, Suh, Hwal, Biomaterials, 23, 1205–1212, (2002).Google Scholar
[11] Hing, KA, Best, SM, Bonfield, W, J Mater Sci Mater Med, 10, 135–145, (1999).Google Scholar