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Polymer Surface Modification Causes Change in Phenotypic Expression of Primary Bone Cells

Published online by Cambridge University Press:  15 February 2011

Sean A. F. Peel ,
R. N. Sodhi ,
Tran Minh Duc  and
John E. Davies
Sean A. F. Peel
Affiliation:
Centre for Biomaterials, University of Toronto, 170 College Street, Toronto, ON, M5S 1A1, Canada.
R. N. Sodhi
Affiliation:
Centre for Biomaterials, University of Toronto, 170 College Street, Toronto, ON, M5S 1A1, Canada.
Tran Minh Duc
Affiliation:
ESCA Centre for Surface Nanoanalysis and Technology, University Claude Bernard Lyon 1, Lyon, France.
John E. Davies*
Affiliation:
Centre for Biomaterials, University of Toronto, 170 College Street, Toronto, ON, M5S 1A1, Canada.
*
#Address for correspondence: J.E. Davies, Centre for Biomaterials, University of Toronto, 170 College Street, Toronto, ON, M5S 1A1, Canada.
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Abstract

Primary rat bone marrow cells were cultured on bacteriological grade polystyrene dishes which had been treated in selected areas with concentrated sulphuric acid. X-ray photoelectron spectroscopy, angle-resolved and imaging modes, and atomic force microscopy showed that the acid treatment brought about both chemical and topographical changes in the substratum surface. While the bone marrow cells attached to both treated and untreated areas of the surfaces of the dishes, the distribution of early mineralized extracellular matrix in these areas was reproducibily different. Thus, using extracellular matrix deposition as a marker, modification of the polymer surface resulted in the cells adhering to treated and untreated areas exhibiting different phenotypes.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 252: Symposium T – Tissue-Inducing Biomaterials , 1991 , 71
Copyright
Copyright © Materials Research Society 1992

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References

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