Hostname: page-component-586b7cd67f-t8hqh Total loading time: 0 Render date: 2024-11-24T13:31:17.806Z Has data issue: false hasContentIssue false
  • English
  • Français

Novel Biologically Inspired Nanostructured Scaffolds for Directing Chondrogenic Differentiation of Mesenchymal Stem Cells

Published online by Cambridge University Press:  21 February 2013

Benjamin Holmes ,
Nathan J. Castro ,
Jian Li  and
Lijie Grace Zhang
Benjamin Holmes
Affiliation:
Department of Mechanical and Aerospace Engineering, The George Washington University, Washington, DC 20052.
Nathan J. Castro
Affiliation:
Department of Mechanical and Aerospace Engineering, The George Washington University, Washington, DC 20052.
Jian Li
Affiliation:
Department of Mechanical and Aerospace Engineering, The George Washington University, Washington, DC 20052.
Lijie Grace Zhang
Affiliation:
Department of Mechanical and Aerospace Engineering, The George Washington University, Washington, DC 20052. Department of Medicine, The George Washington University, Washington, DC 20052.
Get access

Abstract

Cartilage defects, which are caused by a variety of reasons such as traumatic injuries, osteoarthritis, or osteoporosis, represent common and severe clinical problems. Each year, over 6 million people visit hospitals in the U.S. for various knee, wrist, and ankle problems. As modern medicine advances, new and novel methodologies have been explored and developed in order to solve and improve current medical problems. One of the areas of investigation is tissue engineering [1, 2]. Since cartilage matrix is nanocomposite, the goal of the current work is to use nanomaterials and nanofabrication methods to create novel biologically inspired tissue engineered cartilage scaffolds for facilitating human bone marrow mesenchymal stem cell (MSC) chondrogenesis. For this purpose, through electrospinning techniques, we designed a series of novel 3D biomimetic nanostructured scaffolds based on carbon nanotubes and biocompatible poly(L-lactic acid) (PLLA) polymers. Specifically, a series of electrospun fibrous PLLA scaffolds with controlled fiber dimension and surface nanoporosity were fabricated in this study. In vitro hMSC studies showed that stem cells prefer to attach in the scaffolds with smaller fiber diameter or suitable nanoporous structures. More importantly, our in vitro differentiation results demonstrated that incorporation of the biomimetic carbon nanotubes and poly L-lysine coating can induce GAG and collagen synthesis that is indicative of chondrogenic differentiations of MSCs. Our novel scaffolds also performed better than controls, which make them promising for cartilage tissue engineering applications.

Keywords

fibertissuepolymer
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1498: Symposium L – Biomimetic, Bio-inspired and Self-Assembled Materials for Engineered Surfaces and Applications , 2013 , pp. 59 - 66
Copyright
Copyright © Materials Research Society 2013 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Langer, R. and Vacanti, J. P., Science 260 (5110), 920–926 (1993).CrossRefGoogle Scholar
Vacanti, J. P. and Langer, R., Lancet 354 Suppl 1, SI32–34 (1999).CrossRefGoogle Scholar
Zhang, L., Hu, J. and Athanasiou, K. A., Crit Rev Biomed Eng 37 (1–2), 1–57 (2009).CrossRefGoogle Scholar
Hutmacher, D. W., Biomaterials 21 (24), 2529–2543 (2000).CrossRefGoogle Scholar
Smith, L. A. and Ma, P. X., Colloids and surfaces. B, Biointerfaces 39 (3), 125–131 (2004).CrossRefGoogle Scholar
Yoshimoto, H., Shin, Y. M., Terai, H. and Vacanti, J. P., Biomaterials 24 (12), 2077–2082 (2003).CrossRefGoogle Scholar
Nair, L. S., Bhattacharyya, S. and Laurencin, C. T., Expert opinion on biological therapy 4 (5), 659–668 (2004).CrossRefGoogle Scholar
Ma, Z., Kotaki, M., Inai, R. and Ramakrishna, S., Tissue engineering 11 (1–2), 101–109 (2005).CrossRefGoogle ScholarPubMed
Thorvaldsson, A., Stenhamre, H., Gatenholm, P. and Walkenström, P., Biomacromolecules 9 (3), 1044–1049 (2008).CrossRefGoogle Scholar
Meng, X., Li, W., Young, F., Gao, R., Chalmers, L., Zhao, M. and Song, B., Journal of visualized experiments: JoVE (60) (2012).Google Scholar
Shim, I. K., Jung, M. R., Kim, K. H., Seol, Y. J., Park, Y. J., Park, W. H. and Lee, S. J., Journal of Biomedical Materials Research Part B: Applied Biomaterials 95B (1), 150–160 (2010).CrossRefGoogle Scholar
Chen, L., Zhu, C., Fan, D., Liu, B., Ma, X., Duan, Z. and Zhou, Y., Journal of Biomedical Materials Research Part A 99A (3), 395–409 (2011).CrossRefGoogle Scholar
Lee, H., Yeo, M., Ahn, S., Kang, D.-O., Jang, C. H., Lee, H., Park, G.-M. and Kim, G. H., Journal of Biomedical Materials Research Part B: Applied Biomaterials 97B (2), 263–270 (2011).CrossRefGoogle Scholar
Yaszemski, M. J., Payne, R. G., Hayes, W. C., Langer, R. S., Aufdemorte, T. B. and Mikos, A. G., Tissue engineering 1 (1), 41–52 (1995).CrossRefGoogle Scholar
Zhang, L. and Webster, T. J., Nanotoday 4 (1), 66–80 (2009).CrossRefGoogle Scholar
Phipps, M. C., Clem, W. C., Grunda, J. M., Clines, G. A. and Bellis, S. L., Biomaterials 33 (2), 524–534 (2012).CrossRefGoogle Scholar
Shin, T. J., Park, S. Y., Kim, H. J., Lee, H. J. and Youk, J. H., Biotechnology letters 32 (6), 877–882 (2010).CrossRefGoogle Scholar
Colter, D. C., Class, R., DiGirolamo, C. M. and Prockop, D. J., Proc Natl Acad Sci U S A 97 (7), 3213–3218 (2000).CrossRefGoogle Scholar
Fang, R., Zhang, E., Xu, L. and Wei, S., Journal of Nanoscience and Nanotechnology 10 (11), 7747–7751 (2010).CrossRefGoogle Scholar
Garg, T., Singh, O., Arora, S. and Murthy, R., Critical reviews in therapeutic drug carrier systems 29 (1), 1–63 (2012).CrossRefGoogle Scholar
Cui, X., Breitenkamp, K., Finn, M. G., Lotz, M. and D'Lima, D. D., Tissue engineering. Part A 18 (11–12), 1304–1312 (2012).CrossRefGoogle Scholar
Perera, J. R., Gikas, P. D. and Bentley, G., Annals of the Royal College of Surgeons of England 94 (6), 381–387 (2012).CrossRefGoogle Scholar
Hogervorst, T., Eilander, W., Fikkers, J. T. and Meulenbelt, I., Clinical orthopaedics and related research (2012).Google Scholar