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Microfabricated Patch-Clamp Array for Neural Mems Applications

Published online by Cambridge University Press:  01 February 2011

Timothy M. Kubow ,
Karen C. Cheung ,
Loren F. Bentley  and
Luke P. Lee
Timothy M. Kubow
Affiliation:
Joint Graduate Group in Bioengineering University of California San Francisco/ Berkeley Department of Bioengineering, University of California, Berkeley Berkeley Sensor and Actuator Center, University of California, Berkeley Berkeley, CA 94720, U.S.A.
Karen C. Cheung
Affiliation:
Joint Graduate Group in Bioengineering University of California San Francisco/ Berkeley Department of Bioengineering, University of California, Berkeley Berkeley Sensor and Actuator Center, University of California, Berkeley Berkeley, CA 94720, U.S.A.
Loren F. Bentley
Affiliation:
Joint Graduate Group in Bioengineering University of California San Francisco/ Berkeley Department of Bioengineering, University of California, Berkeley Berkeley Sensor and Actuator Center, University of California, Berkeley Berkeley, CA 94720, U.S.A.
Luke P. Lee
Affiliation:
Joint Graduate Group in Bioengineering University of California San Francisco/ Berkeley Department of Bioengineering, University of California, Berkeley Berkeley Sensor and Actuator Center, University of California, Berkeley Berkeley, CA 94720, U.S.A.
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Abstract

Microfabricated patch clamping devices comprising planar arrays of individually addressable nozzles, fluidic channels and electrodes have been developed. Patch clamp based electrophysiological techniques are among the most widespread methods in neurophysiology and are used to address a broad range of cellular physiology and quantitative biological questions. Among the limitations of the technique are the difficulty of obtaining multiple patches on connected cells or on the same cell, limited stability of patches, and constraints on chemical and optical access to the patched membrane. The parallel array device will enable the formation of multiple seals simultaneously. The structure facilitates visualization of the interior of the patched membrane during electrical recording, as well as delivery of chemicals. The microfabrication technique gives precise control over the capacitive and resistive characteristics of the electrode channels, as well as the flow resistance, which are important factors in patch clamp recording. The device is fabricated using an SOI wafer and Deep Reactive Ion Etching to create an array of cylindrical nozzles, each of which has a core of silicon dioxide and interior walls of silicon nitride. Vertical channel segments and plumbing holes are fabricated by deep reactive ion etching through the wafer. Important electrical properties of the device were characterized, and patch clamping was attempted.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 729: Symposium U – MEMS and BioMEMS , 2002 , U4.12
Copyright
Copyright © Materials Research Society 2002

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References

1. Neher, E., Sakmann, B., Nature 260, 5554 (1976).Google Scholar
2. Sakmann, B., Neher, E., Eds., Single-Channel Recording, (Plenum Press, New York, 1983).Google Scholar
3. Connolly, J., Kennedy, C., Journal of Receptor and Signal Transduction Research 21, 21 (2001).Google Scholar
4. Fertig, N., Tilke, A., Blick, R. H., Kotthaus, J. P., Behrends, J. C., and Bruggencate, G. ten, Applied Physics Letters 77, 8 (2000).Google Scholar
5. Schmidt, C., Mayer, M., and Vogel, H., Angewandte Chemie International Edition 39, 17, (2000).Google Scholar
6. Fertig, N., Meger, Ch., Blick, R. H., Trautmann, Ch., and Behrends, J. C., Physical Review E 64, 4 Google Scholar
7. Goodman, M. B., Lockery, S. R., Journal of Neuroscience Methods 100, 1–2 (2000).Google Scholar
8. Levis, R. A., Rae, J. L., Biophysical Journal 65, 4 (1993).Google Scholar