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Microfabricated Device for Impedance-Based Detection of Bacterial Metabolism

Published online by Cambridge University Press:  01 February 2011

Rafael Gómez ,
Michael R. Ladisch ,
Arun K. Bhunia  and
Rashid Bashir
Rafael Gómez
Affiliation:
School of Electrical and Computer Engineering
Michael R. Ladisch
Affiliation:
Department of Agricultural and Biological Engineering
Arun K. Bhunia
Affiliation:
Department of Food Science
Rashid Bashir
Affiliation:
School of Electrical and Computer Engineering Department of Biomedical Engineering Purdue University, West Lafayette, Indiana 47907, U.S.A.
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Abstract

We present the use of a microfabricated device for impedance-based detection of a few live bacterial cells. Impedance-based detection relies on measuring changes in the AC impedance of two electrodes immersed in a liquid were the bacteria are cultured, caused by the release of ionic species by metabolizing bacterial cells. Rapid detection of a few cells (1 to 10) is possible if the cells are confined into a volume on the order of nanoliters. A microfluidic biochip prototype has been fabricated to test this miniaturized assay. The conductance of the bacterial suspensions is extracted from measuring their complex impedance in a 5.27 nl chamber in the biochip, at several frequencies between 100 Hz and 1 MHz. Measurements on suspensions of the bacteria Listeria innocua, Listeria monocytogenes, and Escherichia coli in a low conductivity buffer demonstrate that, under the current experimental conditions, the minimum detection level is between 50 and 200 live cells, after two hours of off-chip incubation. Work is in progress to develop techniques for selective capture of bacteria inside the chip, and to minimize background changes in impedance during on-chip incubation.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 729: Symposium U – MEMS and BioMEMS , 2002 , U4.6
Copyright
Copyright © Materials Research Society 2002

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