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Label-free Electrochemical Biosensor Based on Graphene/Ionic Liquid Nanocomposite for the Detection of Organophosphate Pesticides

Published online by Cambridge University Press:  07 March 2011

Tae Jung Park ,
MinHo Yang ,
Bong Gill Choi ,
Nam Su Heo ,
Seok Jae Lee ,
Won Hi Hong  and
Sang Yup Lee
Tae Jung Park
Affiliation:
BioProcess Engineering Research Center, Center for Systems & Synthetic Biotechnology, and Institute for the BioCentury, KAIST, Daejeon 305-701, Republic of Korea
MinHo Yang
Affiliation:
Department of Chemical & Biomolecular Engineering (BK21 program), KAIST, Daejeon 305-701, Republic of Korea
Bong Gill Choi
Affiliation:
Department of Chemical & Biomolecular Engineering (BK21 program), KAIST, Daejeon 305-701, Republic of Korea
Nam Su Heo
Affiliation:
BioProcess Engineering Research Center, Center for Systems & Synthetic Biotechnology, and Institute for the BioCentury, KAIST, Daejeon 305-701, Republic of Korea
Seok Jae Lee
Affiliation:
NTD&I Team, National Nanofab Center, Daejeon 305-806, Republic of Korea
Won Hi Hong
Affiliation:
Department of Chemical & Biomolecular Engineering (BK21 program), KAIST, Daejeon 305-701, Republic of Korea
Sang Yup Lee
Affiliation:
BioProcess Engineering Research Center, Center for Systems & Synthetic Biotechnology, and Institute for the BioCentury, KAIST, Daejeon 305-701, Republic of Korea Department of Chemical & Biomolecular Engineering (BK21 program), KAIST, Daejeon 305-701, Republic of Korea Department of Bio & Brain Engineering, Department of Biological Sciences, and Bioinformatics Research Center, KAIST, Daejeon 305-701, Republic of Korea
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Abstract

The advanced electrodes for detecting organophosphate pesticides were prepared by modification of the gold (Au) electrode with the reduced graphene oxide/ionic liquid (RGO/IL) nanohybrids. Due to the cationic and anionic parts, the ILs on RGO sheets provide the amount of functional groups for dispersion of hybrids and immobilization of organophosphorus hydrolase (OPH) enzymes. After the immobilization of OPH on the RGO/IL-modified Au electrodes, the modified electrodes represent faster electron transfer than that of Au electrode, resulting in high performance of biosensor with response time (~ 10 s) and sensitivity (4.56 nA μM−1). In addition, the OPH/RGO/IL-modified Au electrode displayed good stability and reproducibility.

Keywords

electrical propertiessol-gelsensor
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1283: Symposium B – Carbon-Based Electronic Devices—Processing, Performance and Reliability , 2011 , mrsf10-1283-b08-05
Copyright
Copyright © Materials Research Society 2011

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References

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