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Investigation of migration behavior of rod-like dsDNA in gel with precisely controlled network structure

Published online by Cambridge University Press:  17 February 2014

Xiang Li ,
Kateryna Khairulina ,
Ung-il Chung  and
Takamasa Sakai
Xiang Li
Affiliation:
Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan
Kateryna Khairulina
Affiliation:
Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan
Ung-il Chung
Affiliation:
Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan
Takamasa Sakai
Affiliation:
Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan
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Abstract

We investigated the migration behavior of rodlike double-stranded DNA (dsDNA) in polymer gels and polymer solutions. Tetra-PEG gel, which has a homogeneous network structure, was utilized as a model system, allowing us to systematically tune the polymer volume fraction and molecular weight of network strand. Although we examined the applicability of the existing models, all the models failed to predict the migration behavior. Thus, we proposed a new model based on the Ogston model, which well explained the experimental data of polymer solutions and gels. The polymer volume fraction determined the maximum mobility, while the network strand governed the size sieving effect. From these results, we conclude that the polymer network with lower polymer volume fraction and smaller network strand is better in terms of size separation. The homogeneous polymer network is vital for understanding of particles’ dynamics in polymer network and a promising material for high-performance size separation.

Keywords

diffusionstructuralsol-gel
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1622: Symposium E – Fundamentals of Gels and Self-Assembled Polymer Systems , 2014 , pp. 169 - 174
Copyright
Copyright © Materials Research Society 2014 

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References

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