Hostname: page-component-78c5997874-m6dg7 Total loading time: 0 Render date: 2024-11-15T11:17:35.976Z Has data issue: false hasContentIssue false
  • English
  • Français

In Vivo Interactions with (Tissue Culture Pretreated) Dermal Sheep Collagen

Published online by Cambridge University Press:  15 February 2011

P. B. van Wachem ,
P. B. van Wachem ,
L. H. H. Olde Damink ,
P. J. Dijkstra ,
J. Feijen  and
P. Nieuwenhuis
P. B. van Wachem
Affiliation:
Department of Histology and Cell Biology, Section Biomaterials Research, University of Groningen, Oostersingel 69/2, 9713 EZ Groningen, The Netherlands.
P. B. van Wachem
Affiliation:
Department of Histology and Cell Biology, Section Biomaterials Research, University of Groningen, Oostersingel 69/2, 9713 EZ Groningen, The Netherlands.
L. H. H. Olde Damink
Affiliation:
Department of Chemical Technology, University of Twente, p.o. Box 217, 7500 AE Enschede, The Netherlands
P. J. Dijkstra
Affiliation:
Department of Chemical Technology, University of Twente, p.o. Box 217, 7500 AE Enschede, The Netherlands
J. Feijen
Affiliation:
Department of Chemical Technology, University of Twente, p.o. Box 217, 7500 AE Enschede, The Netherlands
P. Nieuwenhuis
Affiliation:
Department of Histology and Cell Biology, Section Biomaterials Research, University of Groningen, Oostersingel 69/2, 9713 EZ Groningen, The Netherlands.
Get access

Abstract

Pretreatment in tissue culture (TC) was previously found to markedly reduce the in vitro cytotoxicity of two types of crosslinked dermal sheep collagens (DSC's). This in vivo study confirms our in vitro results, in that TC-pretreatment of crosslinked DSC's resulted in the marked reduction or elimination of cytotoxic effects, such as increased cell infiltration, a deviant neutrophil-morphology, lipid formation and cell death. TC-pretreatment affected the crosslinked state of both DSC's in a different way, which could be deduced from the differences in gelatin-formation and presence of giant cells from macrophage- or fibroblast-origin. The results are explained in view of the differences in crosslinking.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 252: Symposium T – Tissue-Inducing Biomaterials , 1991 , 117
Copyright
Copyright © Materials Research Society 1992

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

1. Chvapil, M., Kronenthal, R.L., Van Winkle, W., Medical and surgical applications of collagen, Int. Rev. Conn. Tissue Res., 6, 1–61, Eds.: Hall, D.A., Jackson, D.S., Academic Press, London, UK, 1973 Google Scholar
2. Huc, A., Collagen biomaterials: characteristics and applications, J. Am. Leather Chem. Assoc. 80,195–212, 1985 Google Scholar
3. Pharriss, B.B., Collagen as a biomaterial, J.Am. Leather Chem. Assoc., 75, 474–485, 1980 Google Scholar
4. Speer, D.P., Chavpil, M., Eskelson, C.D. and Ulreich, J., Biological effects of residual glutaraldehyde in glutaraldehyde tanned collagen biomaterials, J. of Biomed. Mat. Res., 14, 753–764, 1980.Google Scholar
5. Ulreich, J.B., Chvapil, M., A quantitative microassay for in-vitro toxicity testing of biomaterials, J. Biomed. Mater. Res., 15, 913–922, 1981 Google Scholar
6. Van Luyn, M.J.A.Van, Wachem, P.B.Olde, Damink, L.Ten, Hoopen, H., Feijen, J., Nieuwenhuis, P., Methylcellulose culture as a new cytotoxicity test system for biomaterials, Mater. in Med. 2, 142–148, 1991 Google Scholar
7. Van Luyn, M.J.A.Van, Wachem, P.B.Olde, Damink, L., Dijkstra, P.J., Feijen, J., Nieuwenhuis, P., Relations between in vitro cytotoxicity and crosslinked dermal sheep collagens, Submitted to J. Biomed. Mater. Res.Google Scholar
8. Van Luyn, M.J.A., Van Wachem, P.B., Jonkman, M.H., Nieuwenhuis, P., Cytotoxicity testing of wound dressings using methylcellulose cell culture, accepted for publication in BiomaterialsGoogle Scholar
9. Van Wachem, P.B., van Luyn, M.J.A., Koerten, H.K.Olde, Damink, L.Ten, Hoopen, H., Feijen, J., Nieuwenhuis, P., In vivo degradation of processed dermal sheep collagen evaluated with transmission electron microscopy, Biomaterials, 12, 215–223, 1991 Google Scholar
10. Van Wachem, P.B.van, Luyn, M.J.A.Olde, Damink, L., Feijen, J., Nieuwenhuis, P., Tissue interactions with dermal sheep collagen implants: a transmission electron microscopical evaluation, Cells and Materials, 1, 3, 251–263, 1991 Google Scholar
11. Van Gulik, T.M., The processing of sheepskin for use as a dermal collagen graft, Neth. J. Surg. 39, 90–94, 1987 Google Scholar
12. Van Gulik, T.M., Christiano, R.A., Broekhuizen, A.H., Raaymakers, E.L.F.B., Klopper, P.J., A tanned sheep dermal collagen graft as a dressing for split-skin graft donor sites, Neth. J. Surg., 41, 65–67, 1989 Google Scholar
13. Hulstaert, C.E., Kalicharan, D., Hardonk, M.J., Cytochemical demonstration of phosphatases in rat liver by a cerium-based method in combination with osmium tetroxide and potassium ferrocyanide postfixation, Histochemistry 78, 71–79, 1983 Google Scholar
14. Yano, E., Yoshioka, M., Koizumi, A., Relationship between chemical structure and cytotoxicity of aliphatic maines examined by a microtiter system with cultured fibroblasts, Jap. J. Ind. Health, 23, 537–543, 1981 Google Scholar
15. Berode, M., Leuenberger, Ph. Savolainen, H., Phenotype-dependent inhibition of human al-antitrypsin by 1,6 hexanediamine in vitro, Biochem. Int. 17, 141–145, 1988 Google Scholar
16. Johannsen, F.R., Levinskas, G.J., Toxicological profile of orally administered 1,6 hexane diamine in the rat, J. Appl. Toxicol. 7, 259–263, 1987 CrossRefGoogle ScholarPubMed
17. Cooke, A., Oliver, R.F., Edward, M., An in vitro cytotoxicity study of aldehyde-treated pig dermal collagen, Br. J. Exp. Path. 64, 172–176, 1983 Google Scholar
18. Eybl, E., Griesmacher, A., Grimm, M.,Wolner, E., Toxic effects of aldehydes released from fixed pericardium on bovine aortic endothelial cells, J. Bomed. Mater. Res. 23, 1355–1365, 1989 CrossRefGoogle ScholarPubMed
19. Huang-Lee, L.L., Cheung, D.T., Nimni, M.E., Biochemical changes and cytotoxicity associated with the degradation of polymeric glutaraldehyde derived crosslinks, J. Biomed. Mater. Res. 24, 1185–1201, 1990 Google Scholar
20. Van der Laan, J.S., van Wachem, P.B., Schakenraad, J.M., Nieuwenhuis, P., Dermal sheep collagen for the repair of abdominal wall defects, J. Artif. Organs, 14, 661–666, 1991 CrossRefGoogle ScholarPubMed