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Hydrogel Composites Containing Carbon Nanobrushes as Tissue Scaffolds

Published online by Cambridge University Press:  14 January 2013

William H. Marks ,
Sze C. Yang ,
George W. Dombi  and
Sujata K. Bhatia
William H. Marks
Affiliation:
Harvard University School of Engineering and Applied Sciences, Cambridge, MA 02138, U.S.A.
Sze C. Yang
Affiliation:
University of Rhode Island, Chemistry Department, Kingston, RI 02881, U.S.A.
George W. Dombi
Affiliation:
University of Rhode Island, Chemistry Department, Kingston, RI 02881, U.S.A.
Sujata K. Bhatia
Affiliation:
Harvard University School of Engineering and Applied Sciences, Cambridge, MA 02138, U.S.A.
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Abstract

The objective of this work is to examine the feasibility of electrically conductive hydrogel composites as scaffolds in tissue engineering and tissue regeneration, and to understand the properties of the composites as a growth matrix for clinically relevant cell lines. The composite is comprised of carbon nanobrushes embedded in a biocompatible poloxamer gel. This work assesses the ability of such composite gels to support the growth of fibroblasts and myocytes and eventually serve as a matrix to stimulate wound closure. In such a model, fibroblasts and myocytes are seeded on the hydrogel and bathed in culture medium. The experimental model assesses the ability of fibroblasts and myocytes to grow into and adhere to the gel. The work demonstrates that carbon nanobrushes can be dispersed within poloxamer gels, and that fibroblasts and myocytes can proliferate within homogenously dispersed carbon nanobrush-containing poloxamer gels. This work also examines the effects of carbon nanobrush content on the rheological properties of the poloxamer gel matrix and shows an improvement in several areas in the presence of carbon nanobrushes. Future work will examine the effects of design parameters such as carbon nanobrush content and matrix structure on wound healing, as well as the growth of tendons and other cell lines within the hydrogel composites. This work has relevance for tissue and cellular engineering and tissue regeneration in clinical medicine.

Keywords

biomaterialcompositenanostructure
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1498: Symposium L – Biomimetic, Bio-inspired and Self-Assembled Materials for Engineered Surfaces and Applications , 2013 , pp. 53 - 58
Copyright
Copyright © Materials Research Society 2013 

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References

REFERENCES

Hunt, NC, Grover, LM. Cell encapsulation using biopolymer gels for regenerative medicine. Biotechnology Letters 2010; 32(6):733–742.CrossRefGoogle ScholarPubMed
Chen, CZC, Raghunath, M. Focus on collagen: in vitro systems to study fibrogenesis and antifibrosis – state of the art. Fibrogenesis Tissue Repair 2009; 2:7.CrossRefGoogle ScholarPubMed
Kawaguchi, M et al. . Preparation of carbon nanotube-alginate nanocomposite gel for tissue engineering. Dental Materials Journal 2006; 25(4):719–725.CrossRefGoogle ScholarPubMed
Basavaraja, C, Kim, BS, Huh, DS. Characterization and AC Electrical Conductivity for the Dispersed Composites Containing Alginate-Multiwalled Carbon Nanotubes. Macromolecular Research 2011; 19(3):233–242.CrossRefGoogle Scholar
Packer, DL, Dombi, GW, Yu, PY, Zidel, P, Sullivan, WG. An in vitro model of fibroblast activity and adhesion formation during flexor tendon healing. The Journal of Hand Surgery 1994; 19(5):769–776.CrossRefGoogle Scholar
Coburn, J, Gibson, M, Bandalini, PA, Laird, C, Mao, HQ, Moroni, L, Seliktar, D, Elisseeff. Biomimetics of the Extracellular Matrix: An Integrated Three-Dimensional Fiber-Hydrogel Composite for Cartilage Tissue Engineering. Smart Structures and Systems 2011; 7(3):213–222.CrossRefGoogle ScholarPubMed
Leor, J, et al. . Bioengineered cardiac grafts: a new approach to repair the infarcted myocardium. Circulation 2000; 102 (III):III56-III61.Google ScholarPubMed
Dvir, T et al. . Prevascularization of cardiac patch on the omentum improves its therapeutic outcome. Proceedings of the National Academy of Sciences USA 2009; 106(35):14990–14995.CrossRefGoogle ScholarPubMed
Bursac, N, Loo, YH, Leong, K, Tung, L. Novel anisotropic engineered cardiac tissues: studies of electrical propagation. Biochemical and Biophysical Research Communications 2007; 361(4):847–853.CrossRefGoogle ScholarPubMed
Dvir, T et al. . Nanowired three-dimensional cardiac patches. Nature Nanotechnology 2011; 6:720–725.CrossRefGoogle ScholarPubMed
Marks, WH, Yang, SC, Dombi, GW, Bhatia, SK. Translational potential for hydrogel composites containing carbon nanobrushes. 38th Annual Northeast Bioengineering Conference (NEBEC) 2012; 392–393.CrossRefGoogle Scholar
Marks, WH, Yang, SC, Dombi, GW, Bhatia, SK, “Interactions of Poloxamer Hydrogel Composites Containing Carbon Nanobrushes With Clinically Relevant Cell Lines,”Proceedings of the ASME 2012 Summer Bioengineering Conference, Fajardo, Puerto Rico, June 2012, SBC2012–80.Google Scholar
Cabana, A, Ait-Kadi, A, Juhasz, J. Study of the Gelation Process of Polyethylene Oxidea-Polypropylene Oxideb-Polyethelene Oxidea Copolymer (Poloxamer 407) Aqueous Solutions. Journal of Colloid and Interface Science 1997; 190:307–312.CrossRefGoogle ScholarPubMed