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Evaluation of Biological Responses of UMR-106 Cells to Porous PHBV Matrix

Published online by Cambridge University Press:  01 February 2011

Dharmaraj Raghavan ,
Hui Liu  and
John Stubbs III
Dharmaraj Raghavan
Affiliation:
[email protected], Howard, Chemistry, 525 College Street, NW, Washington DC, DC, 20059, United States
Hui Liu
Affiliation:
[email protected], Howard University, Washington DC, DC, 20059, United States
John Stubbs III
Affiliation:
[email protected], Howard University, Washington DC, DC, 20059, United States
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Abstract

This paper compared the results of osteoblast-like UMR-106 cells response to 3-D biodegradable porous with nonporous PHBV (poly (3-hydroxybutyrate-co-3-hydroxyvalerate) films. Nonporous PHBV films were prepared by solvent casting and evaporation. Porous PHBV films were prepared by solute leaching of salt/PHBV cast film. Sieved sodium chloride (∼150 μm) was used to create a matrix with high porosity and then leached out by the solvent. Thermo gravimetric analysis showed that over 99% of the salts were leached out by the solvent. Osteoblast-like UMR-106 cells were seeded onto the nonporous and porous PHBV films, respectively. After 6 and 10 days of incubation, growth rate of UMR-106 cells on porous film was higher than on nonporous film. The viable cell proliferation on the porous and nonporous PHBV film was quantified by (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt) (MTS) assay. The results of this study suggest that the UMR-106 cells proliferate more on the porous PHBV matrix and porous PHBV films are a promising material for bone tissue engineering applications.

Keywords

solvent castingbiomaterialporosity
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 950: Symposium D – Biosurfaces and Biointerfaces , 2006 , 0950-D15-06
Copyright
Copyright © Materials Research Society 2007

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References

REFERENCES

1. Mezzenga, R, Ruokolainen, J, Fredrickson, GH, Kramer, EJ, Moses, D, Heeger, AJ, et al. Templating organic semiconductors via self-assembly of polymer colloids. Science (Washington, DC, United States) 2003;299(5614):1872–1874.Google Scholar
2. Yang, H, Lopina, ST. Extended release of a novel antidepressant, venlafaxine, based on anionic polyamidoamine dendrimers and poly(ethylene glycol)-containing semi interpenetrating networks. Journal of Biomedical Materials Research, Part A 2005;72A(1):107–114.Google Scholar
3. Koese, GT, Korkusuz, F, Oezkul, A, Soysal, Y, Oezdemir, T, Yildiz, C, et al. Tissue engineered cartilage on collagen and PHBV matrices. Biomaterials 2005;26(25):5187–5197.Google Scholar
4. Chen G-Q, Wu, Q. The application of polyhydroxyalkanoates as tissue engineering materials. Biomaterials 2005;26(33):6565–6578.Google Scholar
5. Carina, Eldser SKaA-CA. Effect of abiotic factors on the degradation of poly(3- hydroxybutyrate-co-3-hydroxyvalerate) in simulated and natural composting environments. Polymer Degradation and Stability 1999 May;64(2):177–183.Google Scholar
6. Tesema, Y, Raghavan, D, Stubbs, J, III. Bone cell viability on collagen immobilized poly(3-hydroxybutrate-co-3-hydroxyvalerate) membrane: Effect of surface chemistry. Journal of Applied Polymer Science 2004;93(5):2445–2453.Google Scholar
7. Gunaratne, LMWK, Shanks, RA. Multiple melting behavior of poly(3-hydroxybutyrateco-hydroxyvalerate) using step- scan DSC. European Polymer Journal 2005;41(12):2980–2988.Google Scholar
8. Sarazin, P, Virgilio, N, Favis, BD. Influence of the porous morphology on the in vitro degradation and mechanical properties of poly(L-lactide) disks. Journal of Applied Polymer Science 2006;100(2):1039–1047.Google Scholar
9. Smith, E, Yang, J, McGann, L, Sebald, W, Uludag, H. RGD-grafted thermoreversible polymers to facilitate attachment of BMP-2 responsive C2C12 cells. Biomaterials 2005 Dec;26(35):7329–7338.Google Scholar
10. Liu, H, Tesema, Y, Stubbs, J III, Raghavan, D. In vitro degradation of porous PHBV film. Polymer Preprints (American Chemical Society, Division of Polymer Chemistry) 2005;46(2):1246–1247.Google Scholar