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Enzyme-Pretreatment Removes In Vitro Cytoxic Effects of Dermal Sheep Collagen

Published online by Cambridge University Press:  15 February 2011

M. J. A. van Luyn ,
P. B. van Wachem ,
L. H. H. Olde Damink ,
P. J. Dijkstra ,
J. Feijen  and
P. Nieuwenhuis
M. J. A. van Luyn
Affiliation:
Department of Histology and Cell Biology, Section Biomaterials Research, University of Groningen, Oostersingel 69/2, 9713 EZ Groningen, The Netherlands.
P. B. van Wachem
Affiliation:
Department of Histology and Cell Biology, Section Biomaterials Research, University of Groningen, Oostersingel 69/2, 9713 EZ Groningen, The Netherlands.
L. H. H. Olde Damink
Affiliation:
Department of Chemical Technology, Twente University, p.o. Box 217, 7500 AE Enschede, The Netherlands.
P. J. Dijkstra
Affiliation:
Department of Chemical Technology, Twente University, p.o. Box 217, 7500 AE Enschede, The Netherlands.
J. Feijen
Affiliation:
Department of Chemical Technology, Twente University, p.o. Box 217, 7500 AE Enschede, The Netherlands.
P. Nieuwenhuis
Affiliation:
Department of Histology and Cell Biology, Section Biomaterials Research, University of Groningen, Oostersingel 69/2, 9713 EZ Groningen, The Netherlands.
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Abstract

We investigated the in vitro cytotoxicity of commercially available hexamethylenediisocyanate-crosslinked dermal sheep collagen (HDSC). HDSC was found to induce medium cytotoxic effects, as measured with methylcellulose cell culture. Apart from primary cytotoxicity, due to direct release of (extractable) cytotoxic products, HDSC was found to contain secondary cytotoxicity, possibly released by enzymatic interactions. In this study we found proof for this hypothesis, by exposing extracted HDSC to enzyme-containing medium. Furthermore we observed, that enzymatic pre-treatment can remove all secondary cytotoxic products, possibly due to detachment of pendants, which are still coupled to fragments of collagen molecules. The possibility of enzymatic pretreatment of HDSC, to obtain a non-cytotoxic/biocompatible material, may be important for in vivo applications.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 252: Symposium T – Tissue-Inducing Biomaterials , 1991 , 167
Copyright
Copyright © Materials Research Society 1992

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References

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