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Enzymatic Biosensors with Integrated Thin Film a-Si:H Photodiodes

Published online by Cambridge University Press:  31 January 2011

Ana Teresa Pereira ,
Virginia Chu ,
Duarte M. F. Prazeres  and
Joao P. Conde
Ana Teresa Pereira
Affiliation:
[email protected], INESC Microsistemas e Nanotecnologias and IN- Institute of Nanoscience and Nanotechnology, Lisbon, Portugal
Virginia Chu
Affiliation:
[email protected], INESC Microsistemas e Nanotecnologias and IN- Institute of Nanoscience and Nanotechnology, Lisbon, Portugal
Duarte M. F. Prazeres
Affiliation:
[email protected], Centro de Engenharia Biológica e Química, IBB – Institute of Biotechnology and Bioengineering, Instituto Superior Técnico, Lisbon, Portugal
Joao P. Conde
Affiliation:
[email protected], INESC Microsistemas e Nanotecnologias and IN- Institute of Nanoscience and Nanotechnology, Lisbon, Portugal
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Abstract

A microfabricated amorphous silicon photodiode is used to detect chemiluminescent and colorimetric horseradish peroxidase (HRP) enzymatic reactions. Detections limits of 1 nM and 1 pM of HRP are obtained for chemiluminescent and colorimetric measurements, respectively, with the reactions carried out in solution volume of 50 μL in polystyrene microwells. Surface-adsorbed HRP can be detected with a limit of 1 fmol.cm-2 by both detection methods. Immunoassays were performed using HRP-labeled antibodies and the detection of specific antibody-antigen molecular recognition is demonstrated both in the plastic well and inside a microfluidic channel. The application of the a-Si:H/HRP system is extended by coupling HRP with oxidase enzyme systems for glucose detection and a sensitivity of 0.1 mmol/L was achieved.

Keywords

fluidicsbiologicaloptoelectronic
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1153: Symposium A – Amorphous and Polycrystalline Thin Film Silicon Science and Technology–2009 , 2009 , 1153-A08-04
Copyright
Copyright © Materials Research Society 2009

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References

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