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Engineered Nanostructured Coatings for Enhanced Protein Adsorption and Cell Growth

Published online by Cambridge University Press:  27 February 2012

Fereydoon Namavar ,
Alexander Rubinstein ,
Renat F. Sabirianov ,
Geoffrey M. Thiele ,
J. Graham Sharp ,
Utsav Pokharel ,
Roxanna M. Namavar  and
Kevin L. Garvin
Fereydoon Namavar
Affiliation:
Department of Orthopaedic Surgery and Rehabilitation, UNMC, Omaha, NE 68198, U.S.A.
Alexander Rubinstein
Affiliation:
Department of Physics, University of Nebraska, Omaha, NE 68182, U.S.A.
Renat F. Sabirianov
Affiliation:
Department of Physics, University of Nebraska, Omaha, NE 68182, U.S.A.
Geoffrey M. Thiele
Affiliation:
Department of Internal Medicine Rheumatology, UNMC, Omaha, NE 68198, U.S.A.
J. Graham Sharp
Affiliation:
Department of Genetics, Cell Biology and Anatomy, UNMC, Omaha, NE 68198, U.S.A.
Utsav Pokharel
Affiliation:
Department of Orthopaedic Surgery and Rehabilitation, UNMC, Omaha, NE 68198, U.S.A.
Roxanna M. Namavar
Affiliation:
Department of Orthopaedic Surgery and Rehabilitation, UNMC, Omaha, NE 68198, U.S.A.
Kevin L. Garvin
Affiliation:
Department of Orthopaedic Surgery and Rehabilitation, UNMC, Omaha, NE 68198, U.S.A.
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Abstract

We designed and produced pure cubic zirconia (ZrO2) ceramic1 coatings by an ion beam assisted deposition (IBAD) with nanostructures comparable to the size of proteins. Our ceramic coatings exhibit high hardness and a zero contact angle with serum. In contrast to hydroxyapatite (HA), nano-engineered zirconia films possess excellent adhesion to all orthopaedic materials. Cell adhesion and proliferation experiments were performed with a bona fide mesenchymal stromal cell line (OMA-AD). Our experimental results indicate that the nano-engineered cubic zirconia is superior in supporting growth, adhesion, and proliferation. Since cell attachment is mediated by adhesive proteins such as fibronectin (FN), to elucidate why cells attach more effectively to our nanostructures, we performed a comparative analysis of adsorption energies of FN fragment using quantum mechanical calculations and Monte Carlo (MC) simulation both on smooth and nanostructured surfaces. We have found that a FN fragment adsorbs significantly stronger on the nanostructured surface than on the smooth surface2.

Keywords

adhesionadsorptionion-beam assisted deposition
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1418: Symposium LL/MM – Gels and Biomedical Materials , 2012 , mrsf11-1418-mm02-04
Copyright
Copyright © Materials Research Society 2012

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References

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