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Effect of Single-walled Carbon Nanotubes Entry into Mammalian Cells

Published online by Cambridge University Press:  25 October 2012

Howard H. Chen ,
Bradly Baer ,
Christopher S. Evans ,
Hannah M. Ponek  and
Michelle Chen
Howard H. Chen
Affiliation:
Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, U.S.A.
Bradly Baer
Affiliation:
Department of Chemistry, Point Loma Nazarene University, San Diego, CA 92106, U.S.A.
Christopher S. Evans
Affiliation:
Department of Chemistry, Point Loma Nazarene University, San Diego, CA 92106, U.S.A.
Hannah M. Ponek
Affiliation:
Department of Physics and Engineering, Point Loma Nazarene University, San Diego, CA 92106, U.S.A.
Michelle Chen
Affiliation:
Department of Physics and Engineering, Point Loma Nazarene University, San Diego, CA 92106, U.S.A.
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Abstract

We reported a mammalian cell-imaging paradigm to study the cellular response to single-walled carbon nanotubes (SWCNTs). Chinese Hamster Ovarian (CHO) cells were exposed to SWCNTs resuspended in physiologically compatible buffer (phosphate buffered saline, PBS), at concentrations ranging from 0 to 50 μg/mL. Upon exposure, we optically imaged the cells in order to (1) visualize the accumulation SWCNTs in cells in real-time; (2) qualitatively and quantitatively assess the morphological changes associated with cellular stress in the presence of SWCNTs; and (3) serially quantify cell survival with highly sensitive bioluminescence-based imaging. Our results showed that the cell survival obtained from optical imaging agreed with that from CellTiter-Glo (CTG) luminescence viability assay. Acute compromise in the CHO cell’s survival rate was observed under high concentrations of SWCNT exposure. The cellular response as a function of SWCNT concentrations, and exposure time was further investigated.

Keywords

biologicalcellular (material form)nanostructure
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1468: Symposium VV – Nanomedicine for Molecular Imaging and Therapy , 2012 , mrss12-1468-vv05-26
Copyright
Copyright © Materials Research Society 2012

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References

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