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Development of Biodegradable Polyphosphazene- Nanohydroxyapatite Composite Nanofibers Via Electrospinning

Published online by Cambridge University Press:  01 February 2011

Subhabrata Bhattacharyya ,
Lakshmi S. Nair ,
Anurima Singh ,
Nick R. Krogman ,
Jared Bender ,
Yaser E. Greish ,
Paul W. Brown ,
Harry R. Allcock  and
Cato T. Laurencin
Subhabrata Bhattacharyya
Affiliation:
Department of Chemistry, The University of Virginia, Charlottesville, VA-22903
Lakshmi S. Nair
Affiliation:
Department of Orthopaedic Surgery, The University of Virginia, Charlottesville, VA-22903
Anurima Singh
Affiliation:
Department of Chemistry, The Pennsylvania State University, PA-16802
Nick R. Krogman
Affiliation:
Department of Chemistry, The Pennsylvania State University, PA-16802
Jared Bender
Affiliation:
Department of Chemistry, The Pennsylvania State University, PA-16802
Yaser E. Greish
Affiliation:
Intercollege Materials Research Laboratory, The Pennsylvania State University, PA, PA-16802
Paul W. Brown
Affiliation:
Intercollege Materials Research Laboratory, The Pennsylvania State University, PA, PA-16802
Harry R. Allcock
Affiliation:
Department of Chemistry, The Pennsylvania State University, PA-16802
Cato T. Laurencin*
Affiliation:
Department of Orthopaedic Surgery, The University of Virginia, Charlottesville, VA-22903 Department of Chemical Engineering, The University of Virginia, Charlottesville, VA-22904 Department of Biomedical Engineering, The University of Virginia, Charlottesville, VA-22908
*
* Corresponding Author Cato T.Laurencin M.D., Ph.D., University Professor, Lillian T.Pratt Distinguished Professor and Chair of Orthopaedic Surgery, Professor of Biomedical and Chemical Engineering, The University of Virginia, 400 Ray C.Hunt Drive, Suite 330, Charlottesville, VA 22903, Office: (434) 243-0250, Fax: (434) 243-0252, Email: [email protected]
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Abstract

Biodegradable polymeric nanofibers are of great interest as scaffolds for tissue engineering and drug delivery due to their extremely high surface area, high aspect ratio and similarity in structure to the extracellular matrix (ECM). Polyphosphazenes due to their synthetic flexibility, wide range of physico-chemical properties, non-toxic and neutral degradation products and excellent biocompatibility are suitable candidates for biomedical applications. The objective of the present study was to develop and evaluate composite nanofibers of a biodegradable polyphosphazene, poly[bis(ethyl alanato)phosphazene] (PNEA) and nanocrystals of hydroxyapatite (nHAp) via electrospinning. A suspension of nHAp in dimethyl formamide (DMF) sonicated with PNEA solution in tetrahydrofuran (THF) was used to develop composite nanofiber matrices via electrospinning at ambient conditions. In the present study the theoretical loading of nHAp was varied from 50%-90% (w/w) to PNEA. The nHAp content (actual loading of nHAp) of the composite nanofibers was determined by gravimetric estimation. The composite nanofibers were characterized by transmission electron microscopy (TEM), gravimetry and energy dispersive X-ray mapping. This study demonstrated the feasibility of developing novel composite nanofibers of biodegradable polyphosphazenes with more than 50% (w/w) loading of nHAp on and within the nanofibers.

Keywords

PolyphosphazenesBone tissue engineeringElectrospinningNanofiberNanohydroxyapatite.
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 845: Symposium AA – Nanoscale Materials Science in Biology and Medicine , 2004 , AA4.2
Copyright
Copyright © Materials Research Society 2005

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References

REFERENCES

1. Laurencin, C.T, Ambrosio, A. A., Borden, M.D. and Cooper, J. A.. “Tissue engineering:Orthopedic applications.” Annu. Rev. Biomed. Engg, ed. Yarmush, M.L. (1999), pp. 19–46.Google Scholar
2. Li, W. J., Laurencin, C. T., Caterson, E. J., Tuan, R. S., and Ko, F. K.. J. Biomed. Mater. Res. 60 [4], 613. (2002).Google Scholar
3. Tan, W., Krishnaraj, R., and Desai, T. A., Tissue Engineering 7, 203–210 (2001).Google Scholar
4. Ma, P. X. and Zhang, R., J. Biomed. Mater. Res. 46, 60–72 (1999).Google Scholar
5. Bhattacharyya, S., Lakshmi, S., Bender, J., Greish, Y. E., Brown, P. W., Allcock, H. R., and Laurencin, C. T., Materials Research Society Symposium Proceedings EXS–1, 157–163 (2004).Google Scholar
6. Nair, L. S., Bhattacharyya, S., and Laurencin, C. T., Expert Opin. on Biol. Therapy 4 (2004), 659–668.Google Scholar
7. Nair, L.S., Bhattacharyya, S., Bender, J.D., Greish, Y.E., Brown, P.W., Allcock, H.R., and Laurencin, C.T.. Biomacromolecules 5, 2212–2220 (2004).Google Scholar
8. Bostrom, M.P.G., Boskey, A., Kaufman, J.K., Einhorn, T.A., “Form and Function of Bone” Orthopaedic basic science: biology and biomechanics of the musculoskeletal system, ed. Buckwalter, J. A.; Einhorn, T. A.; Simon, S.R. (American academy of orthopaedic surgeons, 2004) pp.319–369.Google Scholar
9. Methods of Tissue Engineering ed. Atala, A., Lanza, R., (Academic Press), (2002).Google Scholar
10. Deng, X., Hao, J., Wang, C., Biomaterials 22(21), 2867–2873 (2001).Google Scholar