Hostname: page-component-78c5997874-j824f Total loading time: 0 Render date: 2024-11-02T21:36:17.275Z Has data issue: false hasContentIssue false
  • English
  • Français

Computer-assisted designing and biofabrication of 3-D hydrogel structures towards thick 3-D tissue engineering

Published online by Cambridge University Press:  24 January 2012

Makoto Nakamura ,
Kenichi Arai ,
Hideki Toda ,
Shintaroh Iwanaga ,
Kozo Ito ,
Capi Genci  and
Toshio Nikaido
Makoto Nakamura
Affiliation:
Graduate school of Science and Engineering for Research, University of Toyama 3190 Gofuku, Toyama 930-8555, Japan E-mail: [email protected]
Kenichi Arai
Affiliation:
Graduate school of Science and Engineering for Research, University of Toyama 3190 Gofuku, Toyama 930-8555, Japan E-mail: [email protected]
Hideki Toda
Affiliation:
Graduate school of Science and Engineering for Research, University of Toyama 3190 Gofuku, Toyama 930-8555, Japan E-mail: [email protected]
Shintaroh Iwanaga
Affiliation:
Graduate school of Science and Engineering for Research, University of Toyama 3190 Gofuku, Toyama 930-8555, Japan E-mail: [email protected]
Kozo Ito
Affiliation:
Graduate school of Science and Engineering for Research, University of Toyama 3190 Gofuku, Toyama 930-8555, Japan E-mail: [email protected]
Capi Genci
Affiliation:
Graduate school of Science and Engineering for Research, University of Toyama 3190 Gofuku, Toyama 930-8555, Japan E-mail: [email protected]
Toshio Nikaido
Affiliation:
Graduate school of Science and Engineering for Research, University of Toyama 3190 Gofuku, Toyama 930-8555, Japan E-mail: [email protected]
Get access

Abstract

Engineering and manufacturing of thick and bio-functional tissue products is one of the big issues in tissue engineering. To produce such tissues, we need some innovative technologies, which enable us to build up thick, three-dimensional structures and to arrange multiple types of cells to make complicated tissue structures. Based on such considerations, we have developed a custom-made inkjet 3D bioprinter, which realized both of direct cell printing and 3D laminating printing with cells and hydrogel. Recently, it has been improved, and here we report recent progresses and our achievements with new version 3D bioprinter.

Image based printing mode and active Z-axis control system were added. As a useful structure, an image of multi-honeycomb pattern was designed in computer and next it was copied and finally in total 100 image data were prepared. Using those digital data, 3D image of thick multi-honeycomb structure was reconstructed in computer, and then, laminating printing was carried out using our new version 3D bioprinter with alginate hydrogel. The new version printer showed good performance of 3D laminating printing and finally complicated 3D multi-honeycomb hydrogel structures could be successfully fabricated. It is indicated that fabrication of cell containing 3D structures based on the computer aided designs is feasible and that such biofabrication technologies must contribute to further innovative advancement of tissue engineering.

Keywords

biomaterialink-jet printingsol-gel
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1415: Symposium HH/II/TT – MEMS, BioMEMS and Bioelectronics–Materials and Devices , 2012 , mrsf11-1415-ii04-01
Copyright
Copyright © Materials Research Society 2012

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

[1] Nakamura, M. Reconstruction of biological three- dimensional tissues: Bioprinting and biofabrication using inkjet technology, in Cell and Organ Printing, Ringeisen, BR, Spargo, BJ, Wu, P eds, Springer, 2010. pp.23–34.Google Scholar
[2] Nakamura, M, Iwanaga, S, Arai, K, Toda, H, Capi, G, Nikaido, T. Computer-Assisted Biofabrication:The challenges on manufacturing 3-D biological tissues for tissue and organ engineering. Proceeding of Symposium on VLSI Technology at Kyoto 2011.06.14 .Google Scholar
[3] Sun, W, Welcome to Biofabrication. Biofabrication 1, 010201, 2009.Google Scholar
[4] Mironov, V, Trusk, T, Kasyanov, V, Little, S, Swaja, R, Markwald, R. Biofabrication: a 21st century manufacturing paradigm, Biofabrication 1(2), 022001–, 2009.Google Scholar
[5] Guillemot, F, Mironov, V, Nakamura, M, Bioprinting is coming of age:, Biofabrication 2, 010201–. 2010.Google Scholar
[6] Nakamura, M, Kobayashi, A, Takagi, F, Watanabe, A, Hiruma, Y, Ohuchi, K, Iwasaki, Y, Horie, M, Morita, I, Takatani, S. Biocompatible inkjet printing technique for designed seeding of individual living cells Tissue Eng. 11, 1658–, 2005 Google Scholar
[7] Nishiyama, Y, Nakamura, M, Henmi, C, Yamaguchi, K, Mochizuki, S, Nakagawa, H, Takiura, K. Development of three-dimensional bio-printer: Construction of cell supporting structures using hydrogel and state-of-the-art inkjet technology. J Biomech Eng 131(3): 035001–, 2009.Google Scholar
[8] Nishiyama, Y, Nakamura, M, Henmi, C, Yamaguchi, K, Mochizuki, S, Nakagawa, H, Takiura, K. Fabrication of 3D cell supporting structures with multi-materials using the bio-printer. Proc. MSEC 2007–31064, 2007 Google Scholar
[9] Henmi, C, Nakamura, M, Nishiyama, Y, Yamaguchi, K, Mochizuki, S, Takiura, K, Nakagawa, H. New approaches for tissue engineering: three dimensional cell patterning using inkjet technology. Inflammation and Regeneration 28(1), 36–40, 2008.Google Scholar
[10] Calvert, P, Printing cells. Science; 318(5848): 208–, 2007.Google Scholar
[11] Nakamura, M, Iwanaga, S, Henmi, C, Arai, K, Nishiyama, Y, Biomatrices and biomaterials for future developments of bioprinting and biofabrication Biofabrication 2 014110–, 2010.Google Scholar
[12] Arai, K, Iwanaga, S, Toda, H, Genci, C, Nishiyama, Y, Nakamura, M, Three-dimensional inkjet biofabrication based on designed images. Biofabrication 3(3):034113–, 2011.Google Scholar