Hostname: page-component-586b7cd67f-dlnhk Total loading time: 0 Render date: 2024-11-25T02:44:11.542Z Has data issue: false hasContentIssue false
  • English
  • Français

Chemo-electrical energy conversion of Adenosine triphosphate in a Biological Ion Transporter

Published online by Cambridge University Press:  01 February 2011

Vishnu Baba Sundaresan ,
Stephen Andrew Sarles ,
Brian J Goode  and
Donald J Leo
Vishnu Baba Sundaresan
Affiliation:
[email protected], Virginia Tech, Mechanical Engineering, 310 Durham Hall, CIMSS, Blacksburg, VA, 24061, United States, 540 2312910, 540 2312910
Stephen Andrew Sarles
Affiliation:
[email protected], Virginia Tech, Mechanical Engineering, 310 Durham Hall, CIMSS, Mechanical Engineering, Virginia Tech, Blacksburg, VA, 24061-0261, United States
Brian J Goode
Affiliation:
[email protected], Virginia Tech, Mechanical Engineering, 310 Durham Hall, CIMSS, Mechanical Engineering, Virginia Tech, Blacksburg, VA, 24061-0261, United States
Donald J Leo
Affiliation:
[email protected], Virginia Tech, Mechanical Engineering, 310 Durham Hall, CIMSS, Mechanical Engineering, Virginia Tech, Blacksburg, VA, 24061-0261, United States
Get access

Abstract

Ion transport across cell membranes happens through protein channels and pumps expending concentration gradients, electrical gradients and energy from chemical reactions. Ion exchange in cell membranes is responsible for nutrient transport from production sites to where they are broken down to release energy. Sucrose transport is vital for growth in higher plants and recent research has led to the discovery of a class of sugar carriers called SUT4. The SUT4 transporter is a low affinity, high capacity proton-sucrose transporter that participates in long distance sucrose transport in higher plants. We demonstrated the possibility to use purified SUT4 transporter proteins — with the genetic code from Arabidopsis thaliana expressed on yeast cells — for fluid transport driven by pH gradient and from exergonic ATP hydrolysis reaction in the presence of ATP-ase enzyme. The SUT4 proteins were reconstituted on a planar bilayer lipid membrane formed from 1-Palmitoyl-2-Oleoyl-sn-Glycero-3-[Phospho-L-Serine] (Sodium Salt) (POPS), 1-Palmitoyl-2-Oleoyl-sn-Glycero- 3-Phosphoethanolamine (POPE) phospholipids on a porous substrate. This article builds upon our previous work to harness energy from the ATP-ase reaction using SUT4 to produce a proton current through SUT4 and demonstrates the technical feasibility to generate electrical current in an external circuit. The results from our characterization experiments on a single cell demonstrate that the power source behaves like a constant current power source with an internal resistance of 10-22 kΩ and produces a peak power of 150 nW.

Keywords

biomimetic (chemical reaction)energetic materialenvironmentally benign
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 949: Symposium C – Smart Dielectric Polymer Properties, Characterization and Their Devices , 2006 , 0949-C02-02
Copyright
Copyright © Materials Research Society 2007

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

1. Truernit, E., 2001. “Plant physiology: The Importance of Sucrose Transporters,” Current Biology 11, pp. R169– R171.Google Scholar
2. Burkle, L., Hibberd, J. M., Quick, W. P., Kühn, C., Hirner, B., and Frommer, W.B., 1998. “The H+-Sucrose cotransporter AtSUT1 is Essential for Sugar Export from Tobacco Leaves”. Plant Physiology, 118(1), pp. 59–68.Google Scholar
3. Weise, A., Barker, L., Kuhn, C., Lalonde, S., Buschmann, H., Frommer, W. B., and Ward, J.M., 2000. “A New Sub-family of Sucrose Transporters, SUT4, with Low Affinity/High Capacity Localized in Enucleate Sieve Elements of Plants”. Plant Cell, 12, August, pp. 1345–1355.Google Scholar
4. Sundaresan, V. B., Leo, D.J., 2006. “Actuation using Protein Transporters driven by Proton Gradients”, Proceedings of IMECE-2006, ASME, Chicago, IL.Google Scholar
5. Sundaresan, V. B., and Leo, D.J., 2006. “Protein-based Microhydraulic Transport for Controllable Actuation”. In Proceedings of SPIE-2006, SPIE Press.Google Scholar
6. Steinem, C., Janshoff, A., Ulrich, W.P., Sieber, M., and Galla, H.J.: Impedance Analysis of Supported Lipid Bilayer Membranes: A Scrutiny of Different Preparation Techniques. Molecular and Cellular Biology Letters 1279: p.169–180 (March 1996)Google Scholar