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Characterizing the effect of substrate stiffness on neural stem cell differentiation

Published online by Cambridge University Press:  29 November 2012

Colleen T. Curley ,
Kristen Fanale  and
Sabrina S. Jedlicka
Colleen T. Curley
Affiliation:
Bioengineering Program, 111 Research Drive, Bethlehem, PA 18015, USA
Kristen Fanale
Affiliation:
Bioengineering Program, 111 Research Drive, Bethlehem, PA 18015, USA
Sabrina S. Jedlicka
Affiliation:
Bioengineering Program, 111 Research Drive, Bethlehem, PA 18015, USA Materials Science and Engineering Department, 5 E. Packer Avenue, Bethlehem, PA 18015, USA Center for Advanced Materials and Nanotechnology, 5 E. Packer Avenue, Bethlehem, PA 18015, USA
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Abstract

Differentiated neurons (dorsal root ganglia and cortical neurons) have been shown to develop longer neurite extensions on softer materials than stiffer ones, but previous studies do not address the ability of neural stem cells to undergo differentiation as a result of material elasticity. In this study, we investigate neuronal differentiation of C17.2 neural stem cells due to growth on polyacrylamide gels of variable elastic moduli. Neurite growth, synapse formation, and mode of division (asymmetric vs. symmetric) were all assessed to characterize differentiation. C17.2 neural stem cells were seeded onto polyacrylamide gels coated with Type I collagen. The cells were then serum starved over a 14 day period, fixed, and analyzed for biochemical markers of differentiation. For division studies, time-lapse imaging of cells on various substrates was performed during serum withdrawal using the Nikon Biostation. Division events were analyzed using ImageJ to quantify sizes of resulting daughter. Data shows that C17.2 cell differentiation (as dictated by number and type of division events) is dependent upon substrate stiffness, with softer polyacrylamide surfaces (140 Pa) leading to increased populations of neurons and increased neurite length. Our data also indicates that the ability of neural stem cells to express synaptic proteins and develop synapses is dependent upon material elasticity.

Keywords

biomaterialbiomedicalelastic properties
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1498: Symposium L – Biomimetic, Bio-inspired and Self-Assembled Materials for Engineered Surfaces and Applications , 2013 , pp. 47 - 52
Copyright
Copyright © Materials Research Society 2012 

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