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Cell Density Dependent Reduction Kinetics of Hexavalent Uranium by Shewanella oneidensis

Published online by Cambridge University Press:  11 February 2011

Lisa Mullen ,
Vanja Klepac ,
Chanathip Pharino ,
Ken Czerwinski  and
Martin Polz
Lisa Mullen
Affiliation:
Department of Nuclear Engineering, Massachusetts Institute of Technology
Vanja Klepac
Affiliation:
Department of Civil and Environmental Engineering, Massachusetts Institute of Technology
Chanathip Pharino
Affiliation:
Department of Civil and Environmental Engineering, Massachusetts Institute of Technology
Ken Czerwinski
Affiliation:
Department of Nuclear Engineering, Massachusetts Institute of Technology
Martin Polz
Affiliation:
Department of Civil and Environmental Engineering, Massachusetts Institute of Technology
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Abstract

Shewanella oneidensis is a widely distributed species of bacteria and is known to utilize several elements such as iron, manganese and sulfur as electron acceptors. In an anoxic environment lacking more electrochemically favourable electron acceptors S. oneidensis is shown to reduce uranium, changing its oxidation state from hexavalent to tetravalent, by the following reaction: H2+ UO22+ → 2H+ + UO2. The uranyl solution concentration (U(VI)) was measured using inductively coupled plasma atomic emission spectroscopy (ICP-AES), and the reduction data were fit to first order. Several cell concentrations were examined and both the rate of uranyl reduction and the total amount of uranyl reduced are found to be dependent upon cell density. The largest rate constant was 0.7 hr-1 corresponding to a cell density of 2.4*109 cells/mL and an initial reduction rate of 1414 μM/hr. A cell concentration of 6.6*108 cells/mL gave rise to an initial reduction rate of 400μM U(VI) per hour, and had, within a period of 72 hours approximately 98% of the original 2 mM uranyl acetate reduced, as opposed to only 87% for 2.4*109 cells/mL.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 757: Symposium II – Scientific Basis for Nuclear Waste Management XXVI , 2002 , II3.12
Copyright
Copyright © Materials Research Society 2003

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