Hostname: page-component-78c5997874-xbtfd Total loading time: 0 Render date: 2024-11-03T00:17:07.316Z Has data issue: false hasContentIssue false
  • English
  • Français

Biomedical Applications of Gold Nanoparticles Functionalized Using Hetero-Bifunctional Poly(ethylene glycol) Spacer

Published online by Cambridge University Press:  01 February 2011

Wei Fu ,
Dinesh Shenoy ,
Jane Li ,
Curtis Crasto ,
Graham Jones ,
Charles Dimarzio ,
Srinivas Sridhar  and
Mansoor Amiji
Wei Fu
Affiliation:
Department of Physics, Northeastern University, Boston, MA 02115
Dinesh Shenoy
Affiliation:
Department of Pharmaceutical Sciences, Northeastern University, Boston, MA 02115
Jane Li
Affiliation:
Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA 02115
Curtis Crasto
Affiliation:
Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA 02115
Graham Jones
Affiliation:
Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA 02115
Charles Dimarzio
Affiliation:
Department of Electrical and Computer Engineering and the Keck Microscope Facility, Northeastern University, Boston, MA 02115
Srinivas Sridhar
Affiliation:
Department of Physics, Northeastern University, Boston, MA 02115
Mansoor Amiji
Affiliation:
Department of Pharmaceutical Sciences, Northeastern University, Boston, MA 02115
Get access

Abstract

To increase the targeting potential, circulation time, and the flexibility of surface-attached biomedically-relevant ligands on gold nanoparticles, hetero-bifunctional poly(ethylene glycol) (PEG, MW 1, 500) was synthesized having a thiol group on one terminus and a reactive functional group on the other. Coumarin, a model fluorescent dye, was conjugated to the PEG spacer and gold nanoparticles were modified with coumarin-PEG-thiol. Surface attachment of coumarin through the PEG spacer decreases the fluorescence quenching effect of gold nanoparticles. The results of cellular cytotoxicity and fluorescence confocal analyses showed that the PEG spacer modified nanoparticles were essentially non-toxic and could be efficiently internalized in the cells within one hour of incubation.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 845: Symposium AA – Nanoscale Materials Science in Biology and Medicine , 2004 , AA5.4
Copyright
Copyright © Materials Research Society 2005

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

1. Zandonella, C. Nature 423: 10–12 (2003).Google Scholar
2. West, J.L. and Halas, N.J.. Curr. Opinion Biotech., 11: 215–217 (2000).Google Scholar
3. Sahoo, S.K. and Labhasetwar, V.. Drug Disc. Today., 8: 1112–1120 (2003).Google Scholar
4. LaVan, D.A., Lynn, D.M., and Langer, R.. Nature Revs., 1: 77–84 (2002).Google Scholar
5. Goodman, C.M., McCusker, C.D., Yilmaz, T., and Rotello, V.M., Bioconj. Chem., 15: 897–900 (2004)Google Scholar
6. Hirsch, L.R., Stafford, R.J., Bankson, J.A., Shersen, S.R., Rivera, B., Price, R.E., Hazle, J.D., Halas, N.J., and West, J.L.. Proc. Natl. Acad. Sci., USA 100: 13549–13553 (2003).Google Scholar
7. Hainfeld, J.F., Slatkin, D.N., and Smilowitz, H.M., Phys. Med. Biol., 49: N309–N315 (2004)Google Scholar
8. Thomas, M and Klibanov, A.. Proc. Natl. Acad. Sci., USA 100: 9138–9143 (2003).Google Scholar
9. Nam, J-M, Thaxton, C.S., and Mirkin, C.A., Science, 301: 1884 (2003).Google Scholar
10. Potineni, A.D.M., Lynn R., Langer and Amiji, M.M.. J. Controlled Rel., 86: 223–234 (2003).Google Scholar
11. Bouzide, A., and Sauve, G.. Org. Lett., 4: 2329–2332 (2002).Google Scholar
12. Fan, C., Wang, S., Hong, J.W., Bazan, G.C., Plaxco, K.W., and Heeger, A.J.. Proc. Natl. Acad. Google Scholar