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Biohybrid Fibro-Porous Vascular Scaffolds: Effect of Crosslinking on Properties

Published online by Cambridge University Press:  18 May 2015

Vinoy Thomas ,
Danna Nozik ,
Harsh Patel ,
Raj K. Singh  and
Yogesh K. Vohra
Vinoy Thomas*
Affiliation:
Department of Materials Science & Engineering, University of Alabama at Birmingham (UAB), BEC 254, 1720 2nd Avenue South, Birmingham, AL 35294, U.S.A. UAB Center for Nanoscale Materials and Biointegration, Birmingham, AL 35294, U.S.A.
Danna Nozik
Affiliation:
Brown University, Providence, RI 02912, U.S.A.
Harsh Patel
Affiliation:
Department of Materials Science & Engineering, University of Alabama at Birmingham (UAB), BEC 254, 1720 2nd Avenue South, Birmingham, AL 35294, U.S.A.
Raj K. Singh
Affiliation:
Vivo Bioscience, Inc. Birmingham, AL 35205, U.S.A.
Yogesh K. Vohra
Affiliation:
UAB Center for Nanoscale Materials and Biointegration, Birmingham, AL 35294, U.S.A.
*
*[email protected]
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Abstract

Tubular grafts were fabricated from blends of polycaprolactone (PCL) and poly(glycolide-co-caprolactone) (PGC) polymers and coated with an extracellular matrix containing collagens, laminin, and proteoglycans, but not growth factors (HuBiogel™). Multifunctional scaffolds from polymer blends and membrane proteins provide the necessary biomechanics and biological functions for tissue regeneration. Two crosslinking agents, a natural crosslinker namely genipin (Gp) and a carbodiimide reagent namely 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC), were used for further stabilizing the protein matrix and the effect of crosslinking was evaluated for structural, morphological, mechanical properties using SEM, DSC and DMA. SEM images and fiber diameter distribution showed fiber-size between 0.2 μm to 1 μm with the majority of fiber diameters being under 500 nm, indicating upper range of protein fiber-sizes (for example, collagen fibers in extracellular matrix are in 50 to 500 nm diameter range). HB coating did not affect the mechanical properties, but increased its hydrophilicity of the graft. Overall data showed that PCL/PGC blends with 3:1 mass ratio exhibited mechanical properties comparable to those of human native arteries (tensile strength of 1-2 MPa and Young’s modulus of <10 MPa). Additionally, the effect of crosslinking on coating stability was investigated to assure the retention of proteins on scaffold for effective cell-matrix interactions.

Keywords

biomaterialpolymerfiber
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1718: Symposium B – Multifunctional Polymeric and Hybrid Materials , 2015 , pp. 79 - 84
Copyright
Copyright © Materials Research Society 2015 

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References

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