Hostname: page-component-78c5997874-j824f Total loading time: 0 Render date: 2024-11-09T16:06:13.188Z Has data issue: false hasContentIssue false
  • English
  • Français

Adsorption of DNA onto Charged Spheres

Published online by Cambridge University Press:  01 February 2011

Alison J. Hodrien ,
Alison M. Voice  and
Thomas A. Waigh
Alison J. Hodrien
Affiliation:
School of Physics and Astronomy, University of Leeds, Leeds, LS2 9JT, UK.
Alison M. Voice
Affiliation:
School of Physics and Astronomy, University of Leeds, Leeds, LS2 9JT, UK.
Thomas A. Waigh
Affiliation:
School of Physics and Astronomy, University of Leeds, Leeds, LS2 9JT, UK.
Get access

Abstract

The complexation behaviour of linear DNA (negatively charged) with surface functionalised sub-micron latex spheres is studied by dynamic light scattering (DLS), small-angle neutron scattering (SANS), small-angle x-ray scattering (SAXS), and micro rheology. The complexes are measured in solution as a function of component concentration (CDNA, CSPH) and added salt concentration (Cs). In the absence of salt, measured radius increases with CDNA (CSPH held constant) up to a plateau value. The presence of salt causes a decrease in the measured complex radius, which may be due either to increased flexibility of the chains allowing them to form a more compact layer on the sphere surface, or dissociation of the two components due to screened electrostatics. More detailed experiments to determine this are underway.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 856: Symposium BB – Multicomponent Polymer Systems-Phase Behavior, Dynamics, and Applications , 2004 , BB10.1
Copyright
Copyright © Materials Research Society 2005

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

1. Netz, R. R. and Joanny, J. F., Macromolecules 32, 9026 (1999).Google Scholar
2. Schiessel, H., Rudnick, J., Bruinsma, R. and Gelbart, W. M., Europhys. Lett. 51, 237 (2000).Google Scholar
3. Schiessel, H., Macromolecules 36, 9 (2003).Google Scholar
4. Mateescu, E. M., Jeppesen, C. and Pincus, P., Europhys. Lett. 46, 493 (1999).Google Scholar
5. Park, S. Y., Bruinsma, R. and Gelbart, W. M., Europhys. Lett. 46, 454 (1999).Google Scholar
6. Nguyen, T. T. and Shklovskii, B. I., J. Chem. Phys. 115, 7298 (2001).Google Scholar