Hostname: page-component-586b7cd67f-t8hqh Total loading time: 0 Render date: 2024-12-01T02:55:33.205Z Has data issue: false hasContentIssue false
  • English
  • Français

Use of Soft Lithography for Multi-layer MicroMolding (MMM) of 3-D PCL Scaffolds for Tissue Engineering

Published online by Cambridge University Press:  17 March 2011

Yang Sun ,
Nicholas Ferrell  and
Derek J. Hansford
Yang Sun
Affiliation:
Biomedical Engineering Center, The Ohio State University, 270 Bevis Hall, 1080 Carmack Rd., Columbus, OH 43210, U.S.A.
Nicholas Ferrell
Affiliation:
Biomedical Engineering Center, The Ohio State University, 270 Bevis Hall, 1080 Carmack Rd., Columbus, OH 43210, U.S.A.
Derek J. Hansford
Affiliation:
Biomedical Engineering Center, The Ohio State University, 270 Bevis Hall, 1080 Carmack Rd., Columbus, OH 43210, U.S.A.
Get access

Abstract

Tissue engineering scaffolds with precisely controlled geometries, particularly with surface features smaller than typical cell dimensions (1-10μm), can improve cellular adhesion and functionality. In this paper, soft lithography was used to fabricate polydimethylsiloxane (PDMS) stamps of arrays of parallel 5μm wide, 5μm deep grooves separated by 45 μm ridges, and an orthogonal grid of lines with the same geometry. Several methods were compared for the fabrication of 3-D multi-layer polycaprolactone (PCL) scaffolds with precise features. First, micromolding in capillaries (MIMIC) was used to deliver the polymer into the small grooves by capillarity; however the resultant lines were discontinuous and not able to form complete lines. Second, spin coating and oxygen plasma were combined to build 3-D scaffolds with the line pattern. The resultant scaffolds had good alignment and adhesion between layers; however, the upper layer collapsed due to the poor mechanical rigidity. Finally, a new multi-layer micromolding (MMM) method was developed and successfully applied with the grid pattern to fabricate 3-D scaffolds. Scanning electron microscopy (SEM) characterization showed that the multi-layered scaffolds had high porosity and precisely controlled 3-D structures.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 823: Symposium W – Biological and Bioinspired Materials and Devices , 2004 , W9.3/O5.3
Copyright
Copyright © Materials Research Society 2004

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1. Curtis, A., Riehle, M.. Phys. Med. Biol. 46, R47 (2001).CrossRefGoogle Scholar
2. Vats, A., Tolley, N.S., Polak, J.M., Clin, J.E.. Otolaryngol. 28(3), 165 (2003).Google Scholar
3. Hutmacher, D.W.. Biomaterials 21, 2529 (2000).CrossRefGoogle Scholar
4. Curtis, A., Wilkinson, C.. Biomaterials 18(24), 1573 (1997).CrossRefGoogle Scholar
5. Freed, L.E., Marquis, J.C., Vunjak-Novakovic, G., Emmanual, J., Langer, R.. Biotechnol. Bioeng. 43, 605 (1994).CrossRefGoogle Scholar
6. Ma, T., Li, Y., Yang, S.T.. Biotechnol. Prog. 15, 715 (1999).CrossRefGoogle Scholar
7. Walboomers, X.F., Jansen, J.A.. Ondotology 89, 2 (2001).Google Scholar
8. Flemming, R.G., Murphy, C.J., Abrams, G.A., Goodman, S.L., Nealey, P.F.. Biomaterials 20, 573 (1999).CrossRefGoogle Scholar
9. Ito, Y.. Biomaterials 20, 2333 (1999).CrossRefGoogle Scholar
10. Curtis, A.S.G., Varde, M.. J. Nat. Cancer. Res. Inst. 33, 15 (1964).Google Scholar
11. Bhatia, S.N., Chen, C.S.. Biomed. Microdevices 2(2), 131 (1999).CrossRefGoogle Scholar
12. Recum, A.F. von, Kooten, T.G. van. J. Biomater. Sci. Polym. Ed. 7, 181 (1995).CrossRefGoogle Scholar
13. Brunette, D.M., Kenner, G.S., Gould, T.R.L.. J. Dent. Res. 62, 1045 (1983).CrossRefGoogle Scholar
14. Clark, P.. Biosens. Bioelec. 9, 657 (1994).CrossRefGoogle Scholar
15. Dalby, M.J., Riehle, M.O., Yarwood, S.J., Wilkinson, C.D.W., Curtis, A.S.G.. Experimental Cell Research 284, 274 (2003).CrossRefGoogle Scholar
16. Folch, A., Mezzour, S., During, M., Hurtado, O., Toner, M., Muller, R.. Biomed. Microdevices 2, 207 (2000).CrossRefGoogle Scholar
17. Desai, T.A.. Medical Engineering & Physics 22, 595 (2000).CrossRefGoogle Scholar
18. Vozzi, G., Flaim, C., Ahluwalia, A., Bhatia, S.. Biomaterials 24, 2533 (2003).CrossRefGoogle Scholar
19. Ward, J.H., Bashir, R., Peppas, N.A.. J. Biomed. Mater. Res. 56, 351 (2001).3.0.CO;2-A>CrossRefGoogle Scholar
20. Liu, V.A., Bhatia, S.N.. Biomed. Microdevices. 4, 257 (2002).CrossRefGoogle Scholar
21. Yan, Y., Xiong, Z., Hu, Y., Wang, S., Zhang, R., Zhang, C.. Materials Letters 57, 2623 (2003).CrossRefGoogle Scholar
22. Janshoff, A., Künneke, S.. European Biophysics Journal 29, 549 (2000).CrossRefGoogle Scholar
23. Li, S.H., Wijn, J.R. De, Layrolle, P., Groot, K. de. J. Biomed. Mater. Res. 61, 109 (2002).CrossRefGoogle Scholar