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Stable, Nanoscale Glycosphingolipid Films for Use in Sensing Applications

Published online by Cambridge University Press:  17 March 2011

Rory Stine ,
Cara-Lynne Schengrund  and
Michael V. Pishko
Rory Stine
Affiliation:
Departments of Chemical Engineering, Chemistry, and Materials Science & Engineering The Pennsylvania State University
Cara-Lynne Schengrund
Affiliation:
Department of Biochemistry and Molecular Biology, Milton Hershey Medical Center, The Pennsylvania State University
Michael V. Pishko*
Affiliation:
Departments of Chemical Engineering, Chemistry, and Materials Science & Engineering The Pennsylvania State University
*
*Author to whom correspondence should be sent: 104 Fenske Laboratory University Park, PA 16802 [email protected]
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Abstract

We have developed a means of producing thin, oriented lipid monolayers which are stable under repeated washing and which may be useful in biosensing or surface- coating applications. Phosphotidylcholine (PC) and the glycosphingolipid (GSL) GM1 were used as representative lipids for this process. Initially, a mixed self-assembled monolayer of octanethiol and hexadecanethiol was constructed on a clean gold surface. This hydrophobic surface was then brought into contact with a thin lipid layer that had been deposited at the air/liquid interface of a solution by evaporating a mixture of lipid in hexane on top of a layer of water. The lipid layer, now deposited on the gold surface, was then heated to cause intercalation of the fatty acid and alkanethiol chains, and cooled to form a highly stable film which withstood repeated rinsing and solution exposure. Presence and stability of the film was confirmed via ellipsometry, FTIR, and QCM, with an average overall thickness of ∼3.5 nm. These films may be potentially useful in biotoxin detection or as a protein resistant layer for the prevention of biofouling.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 823: Symposium W – Biological and Bioinspired Materials and Devices , 2004 , W12.2
Copyright
Copyright © Materials Research Society 2004

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References

(1) Song, X.; Swanson, B. Analytical Chemistry 1999, 71, 2097–2107.CrossRefGoogle Scholar
(2) Gustafson, I. Colloids and Surfaces B 2003, 30, 13–24.CrossRefGoogle Scholar
(3) Spangler, B.; Tyler, B. Analytica Chimica Acta 1999, 399, 51–62.CrossRefGoogle Scholar
(4) Berg, J.; Tymoczko, J.; Stryer, L. Biochemistry, 5th ed.; W. H. Freeman and Company: New York, 2002.Google Scholar
(5) Tang, Z.; Jing, W.; Wang, E. Langmuir 2000, 16, 1696–1702.CrossRefGoogle Scholar
(6) Brechling, A.; Sundermann, M.; Kleinberg, U.; Heinzmann, U. Thin Solid Films 2003, 433, 281–286.Google Scholar
(7) Meuse, C.; Krueger, S.; Majkrzak, C.; Dura, J.; Fu, J.; Connor, J. Biophysical Journal 1998, 74, 1388–1398.CrossRefGoogle Scholar
(8) Hepel, M. Journal of Electroanalytical Chemistry 2001, 509, 90–106.CrossRefGoogle Scholar
(9) Buoninsegni, F.; Becucci, L.; Moncelli, M.; Guidelli, R. Journal of Electroanalytical Chemistry 2001, 500, 395–407.CrossRefGoogle Scholar
(10) Vikholm, I.; Albers, W. Langmuir 1998, 14, 3865–3872.CrossRefGoogle Scholar
(11) Zhang, Y.; Telyatnikov, V.; Sathe, M.; Zeng, X.; Wang, P. Journal of the American Chemical Society 2003, 125, 9292–9293.CrossRefGoogle Scholar
(12) Cremer, P.; Boxer, S. Journal of Physical Chemistry B 1999, 103, 2554–2559.CrossRefGoogle Scholar
(13) Ross, E.; Spratt, T.; Sanchao, L.; Rozanski, L.; O'Brien, D.; Saavedra, S. Langmuir 2003, 19, 1766–1774.CrossRefGoogle Scholar
(14) Ross, E.; Bondurant, B.; Spratt, T.; Conboy, J.; O'Brien, D.; Saavedra, S. Langmuir 2001, 17, 2305–2307.CrossRefGoogle Scholar
(15) Nakamura, T.; Aoki, K.; Chen, J. Electrochimica Acta 2002, 47, 2407–2411.CrossRefGoogle Scholar
(16) Nakamura, T.; Aoki, K.; Chen, J. Electrochemistry Communications 2002, 4, 521–526.CrossRefGoogle Scholar
(17) Facci, P., D. A., , Andolfi, L., Schnyder, B., Kotz, R. Surface Science 2002, 504, 282–292.CrossRefGoogle Scholar
(18) Berzina, T.S., V. I. T., , Petrigliano, A., Alliata, D., Tronin, A.Yu., Nicolini, C. Thin Solid Films 1996, 284–285.Google Scholar
(19) Spangler, B.; Wilkinson, E.; Murphy, J.; Tyler, B. Analytica Chimica Acta 2001, 444, 149–161.CrossRefGoogle Scholar