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Raman spectra of CdSe/ZnS quantum dots bioconjugated to ovarian cancer antibodies

Published online by Cambridge University Press:  19 March 2012

A.L. Quintos Vazquez ,
T. V. Torchynska  and
L. Shcherbyna
A.L. Quintos Vazquez
Affiliation:
ESIME – Instituto Politécnico Nacional, av.IPN, México D. F. 07738, México.
T. V. Torchynska
Affiliation:
ESFM – Instituto Politécnico Nacional, av.IPN, México D. F. 07738, México.
L. Shcherbyna
Affiliation:
V. Lashkarev Institute of Semiconductor Physics at NASU, av. Nauky 45, Kiev, 03028, Ukraine.
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Abstract

This paper presents the analysis of Raman scattering spectra of CdSe/ZnS QDs covered by the amine-derivatized polyethylene glycol (PEG) polymer with and without bioconjugation to bio-molecules: mouse ovarian cancer (OC 125) antibodies (mab). Commercial CdSe/ZnS QDs used in the study are characterized by the color emission with the maximum at 525 nm (2.36 eV) at 300K. Samples of CdSe/ZnS QDs (bio-conjugated and non -conjugated) in the form of an 5 mm-size spot were dried on a polished surface of crystalline Si substrate to ensure a low level of light scattering background.

Raman scattering spectra of non-conjugated QDs can be presented as a superposition of Raman lines: 212.2, 222.5, 308.3, 440.3, 521.0, 618.0, 667.8 943.5, 986.7, cm-1 related to the CdSe core and silicon substrate. The Raman lines 1003.9, 1317.8, 1452.9, 1656.8, 2870.4, 2931.8 and 3059.9 cm-1 deal with the vibration of COH, CH and OH groups of polymer, which covered QDs, were detected additionally. It is revealed that the QD bio-conjugation to the OC 125 antibodies is accompanied with the changes in the intensity of all types of Raman lines: related to the CdSe core, silicon substrate and polymer groups. The explanation of bioconjugation effects has been proposed and discussed.

Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1376: Symposium S11 – Biomaterials for Medical Applications , 2012 , imrc11-1376-s11-p25
Copyright
Copyright © Materials Research Society 2012

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