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New Atomic Force Microscopy Based Astrocyte Cell Shape Index

Published online by Cambridge University Press:  20 March 2013

Volkan Mujdat Tiryaki ,
Usienemfon Adia-Nimuwa ,
Steven Allen Hartz ,
Kan Xie ,
Virginia M. Ayres ,
Ijaz Ahmed  and
David I. Shreiber
Volkan Mujdat Tiryaki
Affiliation:
Department of Electrical and Computer Engineering, Michigan State University, East Lansing, MI 48824, USA
Usienemfon Adia-Nimuwa
Affiliation:
Department of Electrical and Computer Engineering, Michigan State University, East Lansing, MI 48824, USA
Steven Allen Hartz
Affiliation:
Department of Electrical and Computer Engineering, Michigan State University, East Lansing, MI 48824, USA
Kan Xie
Affiliation:
Department of Electrical and Computer Engineering, Michigan State University, East Lansing, MI 48824, USA
Virginia M. Ayres
Affiliation:
Department of Electrical and Computer Engineering, Michigan State University, East Lansing, MI 48824, USA
Ijaz Ahmed
Affiliation:
Department of Biomedical Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA
David I. Shreiber
Affiliation:
Department of Biomedical Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA
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Abstract

A new three-dimensional cell shape index using the AFM height images of cells cultured on cell substrates was defined. The new cell shape index revealed quantitative cell spreading information of cells that is not included in the conventional cell shape index. The quantitative morphometry of rat cerebral cortical astrocytes cultured on four different kinds of cell substrates were investigated using the conventional and the new cell shape index, and the results were compared. The new cell shape index showed the quantitative astrocyte spreading and stellation behavior that agrees with the AFM height images of astrocytes.

Keywords

biomaterialmorphologyscanning probe microscopy (SPM)
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1527: Symposium UU – Scanning Probe Microscopy—Frontiers in Nanotechnology , 2013 , mrsf12-1527-uu05-08
Copyright
Copyright © Materials Research Society 2013

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References

REFERENCES

Matsutani, S. and Yamamoto, N., Glia, 20, 1, (1997).3.0.CO;2-E>CrossRefGoogle Scholar
Vartanian, K. B., Kirkpatrick, S.J., Hanson, S.R., Hinds, M.T., Biochemical and Biophysical Research Communications 371, 787, (2008).CrossRefGoogle Scholar
Moshayedi, P., Costa, L.d.F., Christ, A., Lacour, S.P., Fawcett, J., Guck, J., Franze, K, J. Phys.: Condens. Matter, 22, 194114, (2010).Google Scholar
Tiryaki, V.M., Ayres, V.M., Khan, A.A., Ahmed, I., Shreiber, D.I., and Meiners, S., Int. J. Nanomedicine, 7, 3891, (2012).CrossRefGoogle Scholar
Meiners, S., Ahmed, I., Ponery, A.S., Amor, N., Ayres, V.M., Fan, Y., Chen, Q. and Babu, A.N., Polymer Int., 56, 1340, (2007).CrossRefGoogle Scholar
Delgado-Rivera, R., Harris, S.L., Ahmed, I., Babu, A.N., Patel, R., Ayres, V.M., Flowers, D.A. and Meiners, S., Matrix Bio. 28, 137, (2009).CrossRefGoogle Scholar
Chvátal, A., Andrová, M., and Kirchhoff, F., J. Anat. 210, 671, (2007).CrossRefGoogle Scholar
Tiryaki, V.M., Khan, A.A., Ayres, V.M.., Scanning, 34, 316 (2012).CrossRefGoogle Scholar
Korchev, Y.E., Gorelik, J., Lab, M.J., Sviderskaya, E.V., Johnston, C.L., Coombes, C.R., Vodyanoy, I., and Edwards, C.R.W.. Biophysical Journal 78, 451, (2000).CrossRefGoogle Scholar
Fardin, M.A., Rossier, O.M., Rangamani, P., Avigan, P.D., Gauthier, N.C., Vonnegut, W., Mathur, A., Hone, J., Iyengarband, R. and Sheetz, M.P.. Soft Matter, 6, 4788, (2010).CrossRefGoogle Scholar
Shao, W., Jin, H., Huang, J., Qiu, B., Xia, R., Deng, Z., Cai, J., Chen, Y., Scanning, 2012. doi: 10.1002/sca.21040. [Epub ahead of print] Google Scholar