Hostname: page-component-586b7cd67f-l7hp2 Total loading time: 0 Render date: 2024-11-28T15:59:37.532Z Has data issue: false hasContentIssue false
  • English
  • Français

Multiplexed Electrical Detection of Single Viruses

Published online by Cambridge University Press:  01 February 2011

Gengfeng Zheng ,
Fernando Patolsky  and
Charles M. Lieber
Gengfeng Zheng
Affiliation:
Department of Chemistry and Chemical Biology, Division of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138
Fernando Patolsky
Affiliation:
Department of Chemistry and Chemical Biology, Division of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138
Charles M. Lieber
Affiliation:
Department of Chemistry and Chemical Biology, Division of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138
Get access

Abstract

We report direct, real-time electrical detection of single virus particles with high selectivity using nanowire field effect transistors. Measurements made with nanowire arrays modified with antibodies for influenza A showed discrete conductance changes characteristic of binding and unbinding in the presence of influenza A but not paramyxovirus or adenovirus. Moreover, simultaneous electrical and optical measurements using fluorescently-labelled influenza A demonstrate conclusively that the conductance changes correspond to binding/unbinding of single viruses at the surface of nanowire devices. In addition, studies of nanowire devices modified with antibodies specific for either influenza or adenovirus show that multiple viruses can be selectively detected in parallel. The possibility of large scale integration of these nanowire devices suggests potential for simultaneous detection of a large number of distinct viral threats at the single virus level.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 828: Symposium A – Semiconductor Materials for Sensing , 2004 , A2.2
Copyright
Copyright © Materials Research Society 2005

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

1. Strauss, E., Strauss, J., Strauss, E. G., Viruses and Human Disease (Academic Press, San Diego, 2001).Google Scholar
2. Storch, G. A., Essential of Diagnostic Virology (Churchill Livingstone, Edinburgh, 1999).Google Scholar
3. Cui, Y., Wei, Q., Park, H., Lieber, C. M., Science 293, 1289 (2001).Google Scholar
4. Hahm, J., Lieber, C. M., Nano Lett. 4, 51 (2004).Google Scholar
5. Chen, R. J. et al., Proc. Natl. Acad. Sci. 100, 4984 (2003).Google Scholar
6. Chen, R. J. et al., J. Am. Chem. Soc. 126, 1563 (2004).Google Scholar
7. Cui, Y., Zhong, Z., Wang, D., Wang, W. U., Lieber, C. M., Nano Lett. 3, 149 (2003).Google Scholar
8. Jin, S. et al., Nano Lett. 4, 915 (2004).Google Scholar
9. Monoclonal anti-hemagglutinin for influenza A was added to a standard virus solution (100 virusesμL) such that the final antibody concentration was 10 μg/ml. The solution was used directly in experiments. Lower antibody concentrations produced partial blockage of the nanowire detector response.Google Scholar
10. Steinfeld, J. I., Francisco, J. S., Hase, W. L., Chemical Kinetics and Dynamics (Prentice Hall, New Jersey, 1998).Google Scholar
11. Herzer, S., Beckett, P., Wegman, T., Moore, P., Life Science News 13, 16 (2003).Google Scholar