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Micro-injection Molded Polymeric Surfaces for the Maintenance of Human Mesenchymal Stem Cells (hMSCs)

Published online by Cambridge University Press:  27 March 2013

Meghan E. Casey ,
John W. Rodgers ,
Courtney E. LeBlon ,
John P. Coulter  and
Sabrina S. Jedlicka
Meghan E. Casey
Affiliation:
Bioengineering,
John W. Rodgers
Affiliation:
Mechanical Engineering and Mechanics,
Courtney E. LeBlon
Affiliation:
Mechanical Engineering and Mechanics,
John P. Coulter
Affiliation:
Mechanical Engineering and Mechanics,
Sabrina S. Jedlicka
Affiliation:
Bioengineering, Materials Science and Engineering, Center for Advanced Materials and Nanotechnology Lehigh University, Bethlehem, PA 18015, USA
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Abstract

In this work, we take advantage of injection molding as a high volume and repeatable method to create surface areas for the growth of human mesenchymal stem cells (hMSCs). Ultraviolet lithography, combined with deep reactive ion etching, was used to generate micro-features over a relatively large surface area of a silicon wafer. The micro-featured silicon wafer was used as a mold insert for the micro-injection molding process to create polystyrene and low density polyethylene surfaces. Micro-geometry was used to alter the effective surface stiffness of the polymer substrate. Created samples were characterized via scanning electron microscopy and tensile testing. hMSCs were seeded onto samples for initial studies. Actin and vinculin were visualized through ICC to compare cytoskeletal elements. Changes in cell morphology were examined using ICC. Results indicate that injection molding of microfeatured substrates is a viable technique to produce surfaces amenable to stem cell growth.

Keywords

biomaterialpolymerbiological
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1499: Symposium N – Precision Polymer Materials―Fabricating Functional Assemblies, Surfaces, Interfaces and Devices , 2013 , mrsf12-1499-n05-136
Copyright
Copyright © Materials Research Society 2013

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References

REFERENCES

Pittenger, M. F., Mackay, A. M., Beck, S. C., Jaiswal, R. K., Douglas, R., Mosca, J. D., Moorman, M. A., Simonetti, D. W., Craig, S., Marshak, D. R., Science 284, 143–147 (1999).CrossRefGoogle Scholar
Stenderup, K., Justesen, J., Clausen, C., Kassem, M., Bone 33, 919–926 (2003).CrossRefGoogle ScholarPubMed
Mets, T. and Verdonk, G., Mech. Ageing Dev. 16, 81–89 (1981).CrossRefGoogle Scholar
Baxter, M. A., Wynn, R. F., Jowitt, S. N., Wraith, J. E., Fairbairn, L. J., Bellantuono, I., Stem Cells 22, 675–682 (2004).CrossRefGoogle Scholar
Maloney, J. M., Nikova, D., Lautenschlager, F., Clarke, E., Langer, R., Guck, J. and Van Vliet, K. J., Biophys. J. 20, 2479–2487 (2010).CrossRefGoogle Scholar
Engler, A. J., Sen, S., Sweeney, H. L. and Discher, D. E., Cell 126, 677–689 (2006).CrossRefGoogle ScholarPubMed
Vogel, V. and Sheetz, M., Nat. Rev. Mol. Cell Biol. 7, 265–275 (2006).CrossRefGoogle Scholar
Fu, J., Wang, Y. K., Yang, M. T., Desai, R. A., Yu, X., Liu, Z. and Chen, C. S., Nat. Methods 7, 733–736 (2010).CrossRefGoogle Scholar
Mrksich, M., Nat. Mater. 10, 559–560 (2011).CrossRefGoogle Scholar
Technical Data: Polyethylene CP 851. A. Schulman.Google Scholar
Jansen, H., deBoer, M., Legtenberg, R. and Elwenspoek, M., J Micromech. Microeng. 5, 115–120 (1995).CrossRefGoogle Scholar
Yoon, S., Cha, N., Lee, J. S., Park, J., Carter, D. J., Mead, J. L. and Barry, C. M. F., Polymer Eng. Sci 50, (2010)CrossRefGoogle Scholar