Hostname: page-component-586b7cd67f-l7hp2 Total loading time: 0 Render date: 2024-11-24T20:38:43.188Z Has data issue: false hasContentIssue false
  • English
  • Français

Microfluidic Cell Volume Biosensor for High Throughput Drug Screening

Published online by Cambridge University Press:  01 February 2011

Daniel A. Ateya ,
Frederick Sachs  and
Susan Z. Hua
Daniel A. Ateya
Affiliation:
Bio-MEMS and Bio-Materials Laboratory, Department of Mechanical and Aerospace Engineering, SUNY-Buffalo, Buffalo, NY 14260, USA
Frederick Sachs
Affiliation:
Hughes Center for Single Molecule Biophysics, Department of Physiology and Biophysics, SUNY-Buffalo, Buffalo, NY 14214, USA
Susan Z. Hua*
Affiliation:
Bio-MEMS and Bio-Materials Laboratory, Department of Mechanical and Aerospace Engineering, SUNY-Buffalo, Buffalo, NY 14260, USA Hughes Center for Single Molecule Biophysics, Department of Physiology and Biophysics, SUNY-Buffalo, Buffalo, NY 14214, USA
*
* Corresponding author: S.Z.H., E-mail: [email protected]
Get access

Abstract

The maintenance of cell volume is critical to health. Cell volume change reflects many biological and physiological processes. We have developed a lab-chip to measure cell volume change in real-time with high sensitivity and resolution, and applicable to both adherent and suspended cell populations. The volume change was detected by measuring the impedance of extra-cellular solution within a microfluidic chamber containing the cells. Using microfabrication to make precise chamber dimensions, volume change can be detected in response to an osmotic gradient <1mOsm. The sensor provides rapid screening of pharmaceutical agents affecting cell volume. We have screened for peptides that affect cell volume regulation and found one in spider venom that inhibits at ∼100pM.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 845: Symposium AA – Nanoscale Materials Science in Biology and Medicine , 2004 , AA5.12
Copyright
Copyright © Materials Research Society 2005

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

[1] Haussinger, D., Biochem. J. 313, 697 (1996).Google Scholar
[2] Sachs, F. Nature Structural Biology 9, 636–637 (2002).Google Scholar
[3] Schliess, F., and Haussinger, D., Bio. Chem. 383, 577 (2002).Google Scholar
[4] Grover, N. B., and Woldringh, C. L., Microbiology-Uk 147, 171 (2001).Google Scholar
[5] Bibby, K. J., and McCulloch, C. A., Am. J. Physiol 266, C1639 (1994).Google Scholar
[6] Gasull, X., et al. Invest Ophthalmol. Vis. Sci 44, 706 (2003).Google Scholar
[7] Crowe, W. E., Altamirano, J., Huerto, L., and varez-Leefmans, F. J., Neuroscience 69, 283 (1995).Google Scholar
[8] Bancroft, A. J., Abel, E. W., Mclaren, M., and Belch, J. J., Platelets. 11, 379 (2000).Google Scholar
[9] Oconnor, E. R., Kimelberg, H. K., Keese, C. R., and Giaever, I., Am. J. Physiol 264, C471 (1993).Google Scholar
[10] Allansson, L., Khatibi, S., Olsson, T., and Hansson, E., J. Neurochem. 76, 472 (2001).Google Scholar
[11] Parkerson, K. A., and Sontheimer, H., GLIA 46, 419 (2004).Google Scholar
[12] Emma, F., McManus, M., and Strange, K., Am. J. Physiol. 272, C1766 (1997).Google Scholar
[13] Hallows, K. R. and Knauf, P. A., in Cellular and Molecular Physiology of Cell Volume Regulation, edited by Strange, K., RCR Press(1994).Google Scholar
[14] Suchyna, T. M., Johnson, J. H., Clemo, H. F., Huang, Z. H., Gage, D. A., Baumgarten, C. M., and Sachs, F., J. Gen. Physiol. 115, 583 (2000).Google Scholar