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Mechanically Stable Free-Standing Bilayer Lipid Membranes in Microfabricated Silicon Chips

Published online by Cambridge University Press:  21 February 2012

Azusa Oshima ,
Ayumi Hirano-Iwata ,
Yasuo Kimura  and
Michio Niwano
Azusa Oshima
Affiliation:
Graduate School of Biomedical Engineering, Tohoku University, 6-6 Aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan
Ayumi Hirano-Iwata
Affiliation:
Graduate School of Biomedical Engineering, Tohoku University, 6-6 Aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan PRESTO, Japan Science and Technology Agency (JST), 4-1-8 Honcho Kawaguchi, Saitama, 332-0012, Japan
Yasuo Kimura
Affiliation:
Laboratory for Nanoelectronics and Spintronics, Research Institute of Electrical Communication, Tohoku University, 2-1-1 Katahira, Sendai, Miyagi, 980-8577, Japan
Michio Niwano
Affiliation:
Graduate School of Biomedical Engineering, Tohoku University, 6-6 Aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan Laboratory for Nanoelectronics and Spintronics, Research Institute of Electrical Communication, Tohoku University, 2-1-1 Katahira, Sendai, Miyagi, 980-8577, Japan
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Abstract

In this paper, we will discuss our recent approaches for improving the mechanical stability of free-standing bilayer lipid membranes (BLMs) by combining with BLM formation and microfabrication techniques. BLMs were prepared across a microaperture fabricated in a silicon (Si) chip and their mechanical stability and electric properties were investigated. BLMs suspended in a thin Si3N4 septum showed a dramatic improvement of BLM stability. The BLMs were resistant to voltage of ±1 V and the membrane lifetime was 15- ~40 h with and without incorporated channels. The membrane containing gramicidin channel exhibited tolerance to repetitive solution exchanges. At first, electric properties of the BLMs, such as noise level and current transient, were necessary to be improved. However, after coating the chip with insulator layers of Teflon and SiO2, total chip capacitance was reduced, leading to noise reduction (1-2 pA in peak-to-peak after low-pass filtering at 1 kHz) and elimination of current transients (< 0.5 ms). Since the vicinity of the aperture edge was remained uncoated, the BLMs formed in the Si chips still showed high mechanical stability after the insulator coatings. The mechanically stable BLMs having electric properties suitable for recording activities of biological channels will open up a variety of applications including high-throughput analysis of ion-channel proteins.

Keywords

biologicaldevicesnanostructure
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1415: Symposium HH/II/TT – MEMS, BioMEMS and Bioelectronics–Materials and Devices , 2012 , mrsf11-1415-ii03-08
Copyright
Copyright © Materials Research Society 2012

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References

REFERENCES

1. Hirano-Iwata, A.; Niwano, M.; Sugawara., M. The design of molecular sensing interfaces with lipid-bilayer assemblies. Trends Anal. Chem., 27, 512–520 (2008).Google Scholar
2. Han, X.; Studer, A.; Sehr, H.; Geissbühler, I.; Berardino, M.D.; Winkler, F. K.; Tiefenauer, L. X. Adv. Mater., 19, 4466–4470 (2007).Google Scholar
3. Hirano-Iwata, A.; Aoto, K.; Oshima, A.; Taira, T.; Yamaguchi, R.; Kimura, Y.; Niwano, M. Free-standing lipid bilayers in silicon chips-membrane stabilization based on microfabricated apertures with a nanometer-scale smoothness. Langmuir, 26, 1949–1952 (2010).Google Scholar
4. Hirano-Iwata, A.; Oshima, A.; Nasu, T.; Kimura, Y.; Niwano, M. Mechanically Stable Lipid Bilayers in Teflon-Coated Silicon Chips for Single-Channel Recordings. Micro- and Nanosystems, in press (2012).Google Scholar
5. White, S.H. Analysis of the torus surrounding planar lipid bilayer membranes. Biophys. J., 12, 432–445 (1972).Google Scholar
6. Andersen, O.S. Ion movement through gramicidin A channels. Biophys. J., 41, 119–133 (1983).Google Scholar
7. Busath, D.D.; Thulin, C.D.; Hendershot, R.W.; Phillips, L.R.; Maughan, P.; Cole, C.D.; Bingham, N.C.; Morrison, S.; Baird, L.C.; Hendershot, R.J.; Cotten, M.; Cross, T.A. Noncontact dipole effects on channel permeation. I. Experiments with (5F-indole) Trp13 gramicidin A channels. Biophys. J., 75, 2830–2844 (1998).Google Scholar
8. Sansom, M. S. P. The biophysics of peptide models of ion channels. Prog. Biophys. Molec. Biol., 55, 139–235 (1991).Google Scholar
9. Mayer, M.; Kriebel, J. K.; Tosteson, M. T.; Whitesides, G. M. Microfabricated Teflon membranes for low-noise recordings of ion channels in planar lipid bilayers. Biophys. J., 85, 2684–2695 (2003).Google Scholar