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Mapping the Micromechanical Properties of Cryo-sectioned Aortic Tissue with Scanning Acoustic Microscopy

Published online by Cambridge University Press:  15 March 2011

Riaz Akhtar ,
Michael J. Sherratt ,
Rachel E.B. Watson ,
Tribikram Kundu  and
Brian Derby
Riaz Akhtar
Affiliation:
Manchester Materials Science Centre, School of Materials, The University of Manchester, Grosvenor Street, Manchester, M1 7HS, United Kingdom
Michael J. Sherratt
Affiliation:
Tissue Injury and Repair Group, Faculty of Medical and Human Sciences, The University of Manchester, 1.581 Stopford Building, Oxford Road, Manchester, M13 9PT, United Kingdom
Rachel E.B. Watson
Affiliation:
Dermatological Sciences Research Group, Faculty of Medical and Human Sciences, The University of Manchester, 1.443 Stopford Building, Oxford Road, Manchester, M13 9PT, United Kingdom
Tribikram Kundu
Affiliation:
Department of Civil Engineering and Engineering Mechanics, University of Arizona, Tucson, Arizona 85721, USA
Brian Derby
Affiliation:
Manchester Materials Science Centre, School of Materials, The University of Manchester, Grosvenor Street, Manchester, M1 7HS, United Kingdom
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Abstract

Although the gross mechanical properties of ageing tissues have been extensively documented, biological tissues are highly heterogeneous and little is known concerning the variation of micro-mechanical properties within tissues. Here, we use Scanning Acoustic Microscopy (SAM) to map the acoustic wave speed (a measure of stiffness) as a function of distance from the outer adventitial layer of cryo-sectioned ferret aorta. With a 400 MHz lens, the images of the aorta samples matched those obtained following chemical fixation and staining of sections which were viewed with fluorescence microscopy. Quantitative analysis was conducted with a frequency scanning or V(f) technique by imaging the tissue from 960 MHz to 1.1 GHz. Undulating acoustic wave speed (stiffness) distributions corresponded with elastic fibre locations in the tissue; there was a decrease in wave speed of around 40 ms-1 from the adventitia (outer layer) to the intima (innermost).

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1132: Symposium Z – Mechanics of Biological and Biomedical Materials , 2008 , 1132-Z03-07
Copyright
Copyright © Materials Research Society 2009

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