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Generating an Intracellular Diffusion Map by Two-Photon Fluorescence Correlation Spectroscopy

Published online by Cambridge University Press:  15 February 2011

P. T. C. So ,
K. Duggar ,
K. M. Berland  and
E. Gratton
P. T. C. So
Affiliation:
ME Dept., MIT, Cambridge, MA 02139, [email protected]
K. Duggar
Affiliation:
ME Dept., MIT, Cambridge, MA 02139, [email protected]
K. M. Berland
Affiliation:
TJ Watson Research Center, IBM, Yorktown Heights, NY
E. Gratton
Affiliation:
LFD, Dept. of Physics, UIUC, Urbana, IL 61801
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Abstract

A new technique for determining cellular viscosity has been developed based on two-photon excitation and fluorescence correlation spectroscopy. We have applied this method to map intracellular diffusion coefficient of small molecules at selected cytoplasmic locations of mouse fibroblast cells. The cytoplasmic diffusion rate is one tenth of that in an aqueous environment.

Keywords

two-photonFCSfluorescence correlation spectroscopycytoplasmic diffusion
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 489: Symposium K – Materials Science of the Cell , 1997 , 187
Copyright
Copyright © Materials Research Society 1998

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